Probing for primary functions of prohibitin in Trypanosoma brucei

Týč, Jiří; Faktorová, Drahomíra; Kriegová, Eva; Jirků, Milan; Vávrová, Zuzana; Maslov, D. A.; Lukeš, Julius

doi:https://dx.doi.org/10.1016/j.ijpara.2009.07.008

Number of the records: 1

Probing for primary functions of prohibitin in Trypanosoma brucei

1.

SYSNO ASEP	0342888
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Probing for primary functions of prohibitin in Trypanosoma brucei
Author(s)	Týč, Jiří (BC-A)_{ORCID, RID} Faktorová, Drahomíra (BC-A)_{RID, ORCID} Kriegová, Eva (BC-A)_SAI Jirků, Milan (BC-A)_RID Vávrová, Zuzana (BC-A) Maslov, D. A. (US) Lukeš, Julius (BC-A)_{RID, ORCID}
Source Title	International Journal for Parasitology. - : Elsevier - ISSN 0020-7519 Roč. 40, č. 1 (2010), s. 73-83
Number of pages	11 s.
Language	eng - English
Country	AU - Australia
Keywords	prohibitin ; mitochondrion ; morphology ; mitochondrial translation
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA204/09/1667 GA ČR - Czech Science Foundation (CSF)
	IAA500960705 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	GP204/06/P423 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000274104300008
DOI	10.1016/j.ijpara.2009.07.008
Annotation	We exploit the tractable reverse genetics of Trypanosoma brucei, the causative agent of African sleeping sickness, in order to specifically analyse the function of PHB in this highly divergent eukaryote. Using inducible RNA interference (RNAi) we show that PHB1 is essential in T brucei, where it is confined to the cell's single mitochondrion forming a high molecular weight complex. PHB1 and PHB2 appear to be indispensible for mitochondrial translation. Their ablation leads to a decrease in mitochondrial membrane potential, however no effect on the level of reactive oxygen species was observed. Flagellates lacking either PHB1 or both PHB1 and PHB2 exhibit significant morphological changes of their organelle, most notably its inflation. Even long after the loss of the PHB proteins, mtDNA was unaltered and mitochondrial cristae remained present, albeit displaced to the periphery of the mitochondrion, which is in contrast to other eukaryotes.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2011

Number of the records: 1