Preparation, production and labelling of scFv against TU-20 and HIV

0191579 - UMG-J 20033041 RIV US eng J - Journal Article
Fábry, Milan - Sedláček, Juraj - Kronrád, L. - Málek, Z. - Viklický, Vladimír
Preparation, production and labelling of scFv against TU-20 and HIV.
Cancer Biotherapy and Radiopharmaceuticals. Roč. 18, č. 2 (2003), s. 285. ISSN 1084-9785. E-ISSN 1557-8852
R&D Projects: GA MŠMT OE67/1
Institutional research plan: CEZ:AV0Z5052915
Keywords : neurotubulin * antibody * labelling
Subject RIV: CE - Biochemistry
Impact factor: 1.841, year: 2003

Monoclonal antibody (mAb) TU 20 against beta-III neurotubulin, a specific component of neural cytoskeleton, is useful for labelling with radioactive nuclides and consequent detection of local levels of neurotubulin or its fragments in patients' tissues. Findings of increased local levels may indicate certain neurodegenerative diseases or an acute damage of neural cells. This work describes production of a recombinant single-chain form (scFv) of mAb TU20, the procedures for its labelling with iodine radionuclides, and assessment of stability and antigen-binding properties of the radiolabelled product in ELISA tests.
Permanent Link: http://hdl.handle.net/11104/0087321