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Transport of ribosomal RNA within the nucleolus
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SYSNO ASEP 0173378 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Transport of ribosomal RNA within the nucleolus Author(s) Staněk, David (UEM-P)
Koberna, Karel (UEM-P)
Pliss, Artem (UEM-P)
Čtrnáctá, Vlasta (UEM-P)
Malínský, Jan (UEM-P) RID, ORCID
Mašata, Martin (UEM-P)
Večeřová, Jaromíra (UEM-P)
Raška, Ivan (UEM-P)Source Title EUREM 12. - Brno : organizator symposia, 2000 Pages s. b 293 Number of pages 1 s. Action EUREM /12./ Event date 09.07.2000-14.07.2000 VEvent location Brno Country CZ - Czech Republic Language eng - English Country CZ - Czech Republic Subject RIV EB - Genetics ; Molecular Biology R&D Projects VS96129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA304/00/P025 GA ČR - Czech Science Foundation (CSF) GA304/00/1481 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z5039906 - UEM-P Annotation In the present study, we have explored the fact that incorporation of BrU into prerRNA did not interfere with pre-rRNA processing (11) and we have investigated the transport of non - isotopically labeled RNA within the nucleolus by means of transmissiion electron microscopy and confocal laser scanning microscopy.Within 20 minutes, BrU - labeled nucleolar RNA moved from the transcription sites in nucleolar DFC to GC. Double localization of bromouridine labeled RNA and fibrillarin revealed that only a ppart of the fibrillarin rich domains were transcriptionally active. Workplace Institute of Experimental Medicine Contact Arzuv Čaryjeva, arzuv.caryjeva@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2001
Number of the records: 1