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Importance of the counterion in optimization of a borate electrolyte system for analyses of anions in samples with complex matrices performed by capillary zone electrophoresis
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SYSNO ASEP 0102300 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Importance of the counterion in optimization of a borate electrolyte system for analyses of anions in samples with complex matrices performed by capillary zone electrophoresis Title Význam protiiontu v optimalizaci borátového elekrolytového systému pro analýzy aniontů ve vzorcích se složitou matricí, pomocí kapilární zónové elekroforézy Author(s) Křivánková, Ludmila (UIACH-O) RID
Březková, M. (CZ)
Gebauer, Petr (UIACH-O) RID, ORCID
Boček, Petr (UIACH-O) RIDSource Title Electrophoresis. - : Wiley - ISSN 0173-0835
Roč. 25, č. 20 (2004), s. 3406-3415Number of pages 10 s. Language eng - English Country DE - Germany Keywords background electrolyte ; borate buffer ; capillary zone electrophoresis Subject RIV CB - Analytical Chemistry, Separation R&D Projects GA203/02/0023 GA ČR - Czech Science Foundation (CSF) IAA4031401 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z4031919 - UIACH-O Annotation Borate buffers are common background electrolytes for analyses of anions in capillary zone electrophoresis. Usually, sodium borate at a given pH is used and this specification seems to be sufficient for a successful analysis. In this paper, we show that free migration of OH-may deteriorate the analysis of a typical anionic analysis of clinical samples due to uncontrolled migration of OH- throughout the systems of analyzed zones and may damage the stacking of anionic analytes of interest. Results of real analyses of human serum samples confirmed the proposed method and proved that substitution of sodium for ammonium in borate buffers offers reliable analyses of clinical samples having chloride as the bulk component Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2005
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