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Anti-tumour necrosis factor-alpha activity in Ixodes ricinus saliva
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SYSNO ASEP 0051261 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Anti-tumour necrosis factor-alpha activity in Ixodes ricinus saliva Title Aktivita proti faktoru nekrotizujícímu nádory ve slinách klíštěte Ixodes ricinus Author(s) Koník, Peter (BC-A)
Slavíková, Veronika (BC-A)
Salát, Jiří (BC-A) RID, ORCID
Řezníčková, Jana (BC-A)
Dvorožňáková, E. (SK)
Kopecký, Jan (BC-A) RIDSource Title Parasite immunology. - : Wiley - ISSN 0141-9838
Roč. 28, č. 12 (2006), s. 649-656Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords Ixodes ricinus ; tick saliva ; TNF Subject RIV EC - Immunology R&D Projects GA524/05/0811 GA ČR - Czech Science Foundation (CSF) LC06009 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) Annotation TNF-alpha plays an important role in the initiation of inflammation. We identified a TNF-alpha-inhibitory activity in the saliva and salivary gland extract (SGE) from partially fed Ixodes ricinus females. Using mouse and human TNF-alpha-specific ELISA, we showed that saliva or SGE reduced the level of detectable cytokine. Both saliva and SGE inhibited the cytotoxic effect of TNF-alpha. Elimination of the TNF-alpha-inhibitory activity in SGE by trypsin digestion demonstrated that the anti-TNF-alpha factor is a protein. FPLC fractionation of SGE showed one peak of TNF-alpha-inhibitory activity corresponding to a protein with estimated molecular mass 23 kDa. The likely mechanism of the inhibitory effect is direct binding of the cytokine. The TNF-alpha-inhibitory molecule seems to play an important role in the anti-inflammatory effect of tick saliva at the tick feeding site, providing a gateway to the host for tick-borne pathogens. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2007
Number of the records: 1