Complete nucleotide sequence and genome analysis of bacteriophage BFK20 — A lytic phage of the industrial producer Brevibacterium flavum

0044701 - ÚMG 2007 RIV US eng J - Journal Article
Bukovska, G. - Klucar, L. - Vlček, Čestmír - Adamovic, J. - Turna, J. - Timko, J.
Complete nucleotide sequence and genome analysis of bacteriophage BFK20 — A lytic phage of the industrial producer Brevibacterium flavum.
[Kompletní nukleotidová sekvence a genomová analýza bakteriofága BFK20, lytického fága průmyslové bakterie Brevibacterium flavum.]
Virology. Roč. 348, č. 1 (2006), s. 57-71. ISSN 0042-6822. E-ISSN 1089-862X
Grant - others:Slovenská akademie věd(SK) VEGA2/5068/25; Science and Technology Assistance Agency(SK) APVT-51-025004
Institutional research plan: CEZ:AV0Z50520514
Keywords : Bacteriophage * Complete genome sequence * Sequence analysis
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.525, year: 2006

Total DNA genome of bacteriophage BFK20, a lytic phage of Brevibacterium flavum CCM251 – L-lysine industrial producer – was sequenced and analyzed. It consists of 42968 base pairs with an overall molar G+C content of 56.2%. Fifty-five potential open reading frames were identified and annotated using various bioinformatics tools. Clusters of functionally related putative genes were defined (structural, lytic, replication and regulatory). To verify the annotation of structural proteins, they were resolved by 2D gel electrophoresis and were submitted to N-terminal amino acid sequencing. Structural proteins identified included the portal and major and minor tail proteins. Based on the overall genome sequence comparison, similarities with other known bacteriophage genomes include primarily bacteriophages from Mycobacterium spp. and some regions of Corynebacterium spp. genomes – possible prophages. Our results support the theory that phage genomes are mosaics with respect to each other.

Sekvence a analýza kompletního genomu dvouvláknového DNA bakteriofága BFK20. Genom je dlouhý 42 968 nukleotidů s obsahem GC párů 56,2%. Identifikovali jsme 55 otevřených čtecích rámců a anotovali jsme je pomocí různých bioinformatických nástrojů.
Permanent Link: http://hdl.handle.net/11104/0137419