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Analysis of glycosylated cardiolipins from thermophilic bacteria using GC-MS and LC-ESI-MS/MS methods
- 1.0584096 - MBÚ 2025 RIV NL eng J - Journal Article
Kyselová, L. - Řezanka, Tomáš
Analysis of glycosylated cardiolipins from thermophilic bacteria using GC-MS and LC-ESI-MS/MS methods.
Journal of Pharmaceutical and Biomedical Analysis. Roč. 238, January 20 (2024), č. článku 115800. ISSN 0731-7085. E-ISSN 1873-264X
Institutional support: RVO:61388971
Keywords : liquid-chromatography * electrospray-ionization * mass-spectrometry * polar lipids * identification * methylation * acids * Thermophilic bacteria * Partially methylated alditol acetates * Glucosyl-cardiolipins * Hydrophilic interaction liquid chromatography * Electrospray mass spectrometry
OECD category: Microbiology
Impact factor: 3.4, year: 2022
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0731708523005691?via%3Dihub
Unusual glucosesubstituted cardiolipins (Glcx-CLs) in three genera of thermophilic bacteria, having more than one glycosidically linked glucose to the hydroxyl of the central glycerol of Glcx-CLs were identified for the first time in thermophilic bacteria of the genera Geobacillus, Meiothermus, and Thermus. The number of glucoses reached up to five units. The structure of glycosidically linked oligosaccharides was determined based on shotgun analysis MS (electrospray highresolution tandem mass spectrometry), partially methylated alditol acetates were identified by GC-MS, both electron ionization (EI) and positive chemical ionization (PCI), hydrophilic interaction liquid chromatography (HILIC) separation and identification of CLs glycosides by high resolution MS-ESI, and digestion by specific glycosidases.
Permanent Link: https://hdl.handle.net/11104/0352117
Number of the records: 1