σE of Streptomyces coelicolor can function both as a direct activator or repressor of transcription

Pospíšil, Jiří; Schwarz, Marek; Ziková, Alice; Vítovská, Dragana; Hradilová, Miluše; Kolář, Michal; Křenková, Alena; Hubálek, Martin; Krásný, Libor; Vohradský, Jiří

doi:https://dx.doi.org//10.1038/s42003-023-05716-y

Number of the records: 1

σE of Streptomyces coelicolor can function both as a direct activator or repressor of transcription

1.

SYSNO ASEP	0580571
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	σE of Streptomyces coelicolor can function both as a direct activator or repressor of transcription
Author(s)	Pospíšil, Jiří (MBU-M)_{ORCID, RID} Schwarz, Marek (MBU-M)_{ORCID, RID} Ziková, Alice (MBU-M) Vítovská, Dragana (MBU-M)_ORCID Hradilová, Miluše (UMG-J) Kolář, Michal (UMG-J)_{RID, ORCID} Křenková, Alena (UOCHB-X)_{ORCID, RID} Hubálek, Martin (UOCHB-X)_{RID, ORCID} Krásný, Libor (MBU-M)_{RID, ORCID} Vohradský, Jiří (MBU-M)_{RID, ORCID}
Article number	46
Source Title	Communications Biology. - : Nature Publishing Group Roč. 7, January 6 (2024)
Number of pages	16 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	σ factors ; proteins ; Streptomyces coelicolor ; gene expression
OECD category	Microbiology
R&D Projects	GF22-06342K GA ČR - Czech Science Foundation (CSF)
	LM2023055 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LX22NPO5103 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Research Infrastructure	ELIXIR CZ III - 90255 - Ústav organické chemie a biochemie AV ČR, v. v. i.
Method of publishing	Open access
Institutional support	MBU-M - RVO:61388971 ; UMG-J - RVO:68378050 ; UOCHB-X - RVO:61388963
UT WOS	001137230500004
EID SCOPUS	85181490781
DOI	/10.1038/s42003-023-05716-y
Annotation	σ factors are considered as positive regulators of gene expression. Here we reveal the opposite, inhibitory role of these proteins. We used a combination of molecular biology methods and computational modeling to analyze the regulatory activity of the extracytoplasmic σE factor from Streptomyces coelicolor. The direct activator/repressor function of σE was then explored by experimental analysis of selected promoter regions in vivo. Additionally, the σE interactome was defined. Taken together, the results characterize σE, its regulation, regulon, and suggest its direct inhibitory function (as a repressor) in gene expression, a phenomenon that may be common also to other σ factors and organisms.
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2025
Electronic address	https://www.nature.com/articles/s42003-023-05716-y

Number of the records: 1