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Gypsum endolithic phototrophs under moderate climate (Southern Sicily): their diversity and pigment composition
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SYSNO ASEP 0578330 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Gypsum endolithic phototrophs under moderate climate (Southern Sicily): their diversity and pigment composition Author(s) Němečková, K. (CZ)
Mareš, Jan (MBU-M) ORCID
Procházková, L. (CZ)
Culka, A. (CZ)
Košek, F. (CZ)
Wierzchos, J. (ES)
Nedbalová, L. (CZ)
Dudák, J. (CZ)
Tymlová, V. (CZ)
Žemlička, J. (CZ)
Kust, A. (US)
Zima, J. (CZ)
Nováková, E. (CZ)
Jehlička, J. (CZ)Article number 1175066 Source Title Frontiers in Microbiology. - : Frontiers Research Foundation - ISSN 1664-302X
Roč. 14, July 6 (2023)Number of pages 21 s. Language eng - English Country CH - Switzerland Keywords mcmurdo dry valleys ; raman-spectroscopy ; microbial colonization ; atacama desert ; in-situ ; scytonemin ; microorganisms ; cyanobacteria ; carotenoids ; communities ; endoliths ; gypsum ; phototrophs ; cyanobacteria ; green algae ; metagenomics OECD category Microbiology R&D Projects LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED2.1.00/19.0392 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 001032976300001 EID SCOPUS 85165259978 DOI 10.3389/fmicb.2023.1175066 Annotation In this study, we used microscopic, spectroscopic, and molecular analysis to characterize endolithic colonization in gypsum (selenites and white crystalline gypsum) from several sites in Sicily. Our results showed that the dominant microorganisms in these environments are cyanobacteria, including: Chroococcidiopsis sp., Gloeocapsopsis pleurocapsoides, Gloeocapsa compacta, and Nostoc sp., as well as orange pigmented green microalgae from the Stephanospherinia clade. Single cell and filament sequencing coupled with 16S rRNA amplicon metagenomic profiling provided new insights into the phylogenetic and taxonomic diversity of the endolithic cyanobacteria. These organisms form differently pigmented zones within the gypsum. Our metagenomic profiling also showed differences in the taxonomic composition of endoliths in different gypsum varieties. Raman spectroscopy revealed that carotenoids were the most common pigments present in the samples. Other pigments such as gloeocapsin and scytonemin were also detected in the near-surface areas, suggesting that they play a significant role in the biology of endoliths in this environment. These pigments can be used as biomarkers for basic taxonomic identification, especially in case of cyanobacteria. The findings of this study provide new insights into the diversity and distribution of phototrophic microorganisms and their pigments in gypsum in Southern Sicily. Furthemore, this study highlights the complex nature of endolithic ecosystems and the effects of gypsum varieties on these communities, providing additional information on the general bioreceptivity of these environments. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2024 Electronic address https://www.frontiersin.org/articles/10.3389/fmicb.2023.1175066/full
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