Proportions of taxa belonging to the gut core microbiome change throughout the life cycle and season of the bark beetle Ips typographus

0578266 - MBÚ 2024 RIV US eng J - Journal Article
Veselská, Tereza - Švec, Karel - Kostovčík, Martin - Peral-Aranega, E. - García Fraile, Paula - Křížková, Barbora - Havlíček, Václav - Saati-Santamaria, Zaki - Kolařík, Miroslav
Proportions of taxa belonging to the gut core microbiome change throughout the life cycle and season of the bark beetle Ips typographus.
FEMS Microbiology Ecology. Roč. 99, č. 8 (2023), č. článku fiad072. ISSN 0168-6496. E-ISSN 1574-6941
R&D Projects: GA ČR(CZ) GA19-09072S
Institutional support: RVO:61388971
Keywords : dendroctonus-ponderosae coleoptera * picea-abies * bacterial communities * vectored fungi * diversity * spruce * plant * phytopathogenicity * visualization * verbenone * bark beetles * core microbiome * DNA and RNA metabarcoding * gut fungal and bacterial community * Ips typographus * seasonality
OECD category: Microbiology
Impact factor: 4.2, year: 2022
Method of publishing: Open access
https://academic.oup.com/femsec/article/99/8/fiad072/7209148?login=true

The European spruce bark beetle, Ips typographus, is a serious pest of spruce forests in Europe, and its invasion and development inside spruce tissues are facilitated by microorganisms. We investigated the core gut bacterial and fungal microbiomes of I. typographus throughout its life cycle in spring and summer generations. We used cultivation techniques and molecular identification in combination with DNA and RNA metabarcoding. Our results revealed that communities differ throughout their life cycle and across generations in proportion of dominantly associated microbes, rather than changes in species composition. The bacteriome consisted mostly of the phylum Gammaproteobacteria, with the most common orders and genera being Enterobacteriales (Erwinia and Serratia), Pseudomonadales (Pseudomonas), and Xanthomonadales. The fungal microbiome was dominated by yeasts (Saccharomycetes-Wickerhamomyces, Kuraishia, and Nakazawaea), followed by Sordariomycetes (Ophiostoma bicolor and Endoconidiophora polonica). We did not observe any structure ensuring long-term persistence of microbiota on any part of the gut epithelium, suggesting that microbial cells are more likely to pass through the beetle's gut with chyme. The most abundant taxa in the beetle's gut were also identified as dominant in intact spruce phloem. Therefore, we propose that these taxa are acquired from the environment rather than specifically vectored between generations.
Permanent Link: https://hdl.handle.net/11104/0347289