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Antibiotic resistance and biofilm-forming ability of a-toxin-positive Clostridium septicum isolates worsen patient prognosis
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SYSNO ASEP 0576018 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Antibiotic resistance and biofilm-forming ability of a-toxin-positive Clostridium septicum isolates worsen patient prognosis Author(s) Kuzma, J. (SK)
Zavala-Meneses, Sofía Guadalupe (MBU-M)
Škultéty, L'udovít (MBU-M) ORCID, RID
Chmelař, D. (CZ)
Ficik, J. (SK)
Palcova, L. (SK)Source Title Acta Pathologica Microbiologica et Immunologica Scandinavica - ISSN 0903-4641
Roč. 131, č. 8 (2023), s. 434-441Number of pages 8 s. Language eng - English Country DK - Denmark Keywords Alpha toxin ; antibiotic resistance ; biofilm ; Clostridium septicum ; flagellin OECD category Microbiology Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 001019829100001 EID SCOPUS 85162856576 DOI 10.1111/apm.13338 Annotation A total of, 78 Clostridium septicum (CLSE) isolates were screened for genes encoding: a-toxin, flagellin, and resistance to vancomycin (VANg). The isolates were also tested for their ability to form biofilm and their antibiotic susceptibility. All isolates were positive for a-toxin and flagellin genes. However, only 19 isolates (24.3%) showed prevalence for VANg. We observed the strongest capacity to form a biofilm (100%) in isolates from patients with oncologic or septic and febrile diagnoses. This percentage was also very high in patients with colitis and gastrointestinal hemorrhage (72.7%). No less than 43 isolates showed antibiotic resistance, and 21 were multidrug-resistant (MDR). Interestingly, our studies showed a correlation between antibiotic resistance and biofilm formation. A statistically significant difference was observed between biofilm-forming MDR isolates and those with low/no biofilm-forming ability. However, the most impressive observation was the correlation with mortality rate. While the overall mortality rate for CLSE infections was 16.7% (13/78), the mortality rate for patients infected with MDR isolates forming biofilm moderately or strongly reached 38.1% (8/21). This number increased even further when only infections with the biofilm-forming VANg-positive isolates were considered (61.5%, 8/13). Therefore, the ability of a VANg-positive CLSE isolate to form a biofilm has been suggested as a biomarker of poor prognosis. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2024 Electronic address https://onlinelibrary.wiley.com/doi/10.1111/apm.13338
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