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Development of novel fluorescent tags for glycosylation analysis by CE/LIF and CE-MS
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SYSNO ASEP 0573238 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Development of novel fluorescent tags for glycosylation analysis by CE/LIF and CE-MS Author(s) Lavická, Jana (UIACH-O) ORCID, RID
Smolková, Denisa (UIACH-O)
Čmelík, Richard (UIACH-O) RID
Duša, Filip (UIACH-O) RID, ORCIDNumber of authors 4 Source Title MSB 2023. -, 2023
S. 1-1Number of pages 1 s. Publication form Online - E Action MSB 2023. International Symposium on Microscale Separations and Bioanalysis /39./ Event date 21.05.2023 - 24.05.2023 VEvent location Florida State University, Tallahassee Country US - United States Language eng - English Country US - United States Keywords CE/LIF ; CE-MS ; glycosylation analysis Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA22-00236S GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 Annotation In this work, we present design, synthesis, and application of new fluorescent tags providing rapid labeling kinetics, high fluorescence quantum yields, and significantly enhanced detectability by MS. The new fluorescent tags based on pyrene, coumarin and/or BODIPY derivatives with various substituents and modifiable linkers were synthetized and the desired modification allowed to tune the fluorescence and MS properties (preferred wavelength and quantum yield, charge). A detailed comparison of the mechanisms of labeling, reductive amination and hydrazone formation chemistries, showed that the labeling yield obtained by hydrazone formation chemistry is almost one order of magnitude higher compared to the reductive amination approach. Furthermore, the undesired formation of multiple products of N-linked glycan labeling by reductive amination was detected when positively charged labels such as aliphatic/aromatic amines containing a quaternary ammonium group were attached. Labeling with different synthetized tags as well as their comparison with the commercially available labels will be demonstrated by analysis maltooligosaccharides and N-linked glycans by CE/LIF and CE-MS. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2024
Number of the records: 1