Composition of raft-like cell membrane microdomains resistant to styrene-maleic acid copolymer (SMA) solubilization

0571281 - ÚMG 2024 RIV NL eng J - Journal Article
Harant, K. - Čajka, Tomáš - Angelisová, Pavla - Pokorná, Jana - Hořejší, Václav
Composition of raft-like cell membrane microdomains resistant to styrene-maleic acid copolymer (SMA) solubilization.
Biophysical Chemistry. Roč. 296, MAY (2023), č. článku 106989. ISSN 0301-4622. E-ISSN 1873-4200
R&D Projects: GA ČR(CZ) GA19-04047S
Institutional support: RVO:67985823 ; RVO:68378050
Keywords : Membrane rafts * SMA copolymer * Proteomics * Lipidomics * Jurkat cell line
OECD category: Biochemistry and molecular biology; Polymer science (FGU-C)
Impact factor: 3.8, year: 2022
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0301462223000406?via%3Dihub

An advantageous alternative to the use of detergents in biochemical studies on membrane proteins are the recently developed styrene-maleic acid (SMA) amphipathic copolymers. In our recent study [1] we demonstrated that using this approach, most T cell membrane proteins were fully solubilized (presumably in small nanodiscs), while two types of raft proteins, GPI-anchored proteins and Src family kinases, were mostly present in much larger (>250 nm) membrane fragments markedly enriched in typical raft lipids, cholesterol and lipids containing saturated fatty acid residues. In the present study we demonstrate that disintegration of membranes of several other cell types by means of SMA copolymer follows a similar pattern and we provide a detailed proteomic and lipidomic characterization of these SMA-resistant membrane fragments (SRMs).
Permanent Link: https://hdl.handle.net/11104/0342543