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Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays
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SYSNO ASEP 0565880 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Influence of Label and Solid Support on the Performance of Heterogeneous Immunoassays Author(s) Makhneva, E. (CZ)
Sklenárová, D. (CZ)
Brandmeier, J. C. (CZ)
Hlaváček, Antonín (UIACH-O) ORCID
Gorris, H. H. (CZ)
Skládal, P. (CZ)
Farka, Z. (CZ)Number of authors 7 Source Title Analytical Chemistry. - : American Chemical Society - ISSN 0003-2700
Roč. 94, č. 47 (2022), s. 16376-16383Number of pages 8 s. Publication form Print - P Language eng - English Country US - United States Keywords assays ; fluorescence ; up-conversion nanoparticles ; immunosensor Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA21-03156S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support UIACH-O - RVO:68081715 UT WOS 000889895000001 EID SCOPUS 85142460861 DOI 10.1021/acs.analchem.2c03543 Annotation Conventional immunochemical methods used in clinical analysis are often not sensitive enough for early-stage diagnosis, resulting in the need for novel assay formats. Here, we provide a detailed comparison of the effect of different labels and solid supports on the performance of heterogeneous immunoassays. When comparing three types of streptavidin-modified labels-horseradish peroxidase, carboxyfluorescein, and photon-upconversion nanoparticles (UCNPs)-UCNPs led to the most sensitive and robust detection of the cancer biomarker prostate-specific antigen. Additionally, we compared the immunoassay formats based on conventional microtiter plates and magnetic microbeads (MBs). In both cases, the highest signal-to-background ratios and the lowest limits of detection (LODs) were obtained by using the UCNP labels. The MB-based upconversion-linked immunosorbent assay carried out with a preconcentration step provided the lowest LOD of 0.46 pg/mL in serum. The results demonstrate that the use of UCNPs and MBs can significantly improve the sensitivity and working range of heterogeneous immunoassays for biomarker detection. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2023 Electronic address https://hdl.handle.net/11104/0337362
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