New cytogenetic data on Caryophyllaeus laticeps and Paracaryophyllaeus gotoi, parasites of evolutionary interest

0557847 - BC 2023 RIV US eng J - Journal Article
Orosová, M. - Marková, A. - Marec, František - Barčák, D. - Brázová, T. - Oros, M.
New cytogenetic data on Caryophyllaeus laticeps and Paracaryophyllaeus gotoi, parasites of evolutionary interest.
Parasitology. Roč. 149, č. 8 (2022), s. 1094-1105. ISSN 0031-1820. E-ISSN 1469-8161
Grant - others:AV ČR(CZ) SAV-21-03
Program: Bilaterální spolupráce
Institutional support: RVO:60077344
Keywords : chromosome aberration * environmental pollution * FISH
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 2.4, year: 2022
Method of publishing: Limited access
https://www.cambridge.org/core/journals/parasitology/article/abs/new-cytogenetic-data-on-caryophyllaeus-laticeps-and-paracaryophyllaeus-gotoi-parasites-of-evolutionary-interest/B5D68E1E3F01AE2EA47B21DAD912A0FF

Caryophyllideans are intestinal parasites of freshwater fishes, occupying a basal position among the ‘true’ tapeworms. We performed detailed cytogenetic analyses of the well- known caryophyllidean species Caryophyllaeus laticeps. For comparison, we also examined for the first time the chromosomes of Paracaryophyllaeus gotoi, a specific parasite of loaches in China. Both species showed a diploid chromosome number of 2n = 20, n = 10m. Chromomycin A3 (CMA3)/diamidino-2-phenylindole (DAPI) staining performed for the
first time in the class Cestoda revealed CMA3 +/DAPI− bands in the pericentromeric regions of the short arms of chromosome pair no. 7 in the karyotype of C. laticeps. Fluorescence
in situ hybridization with the 18S rDNA probe confirmed the presence of a single cluster of major rDNA near the centromere on a pair of small chromosomes in both species.
These findings support the hypothesis that the ancestral state in the family Caryophyllaeidae is a single interstitial cluster of major rDNA genes and thus one nucleolar organizer region per haploid genome. Our results, which we presented together with literature data plotted on a phylogenetic tree, show stability of caryophyllidean karyotypes at the genus level, but showed differences between genera without a clear phylogenetic signal. The data allowed us to at least formulate a hypothesis about the ancestral haploid chromosome number
of n = 10 for the family Caryophyllaeidae and possibly for the sister family Capingentidae. In addition, we compared two populations of C. laticeps from water bodies with different levels of
polychlorinated biphenyl contamination, showing a slightly increased incidence of chromo- somal abnormalities at the contaminated site.
Permanent Link: https://hdl.handle.net/11104/0339240