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Desiccation as a Post-maturation Treatment Helps Complete Maturation of Norway Spruce Somatic Embryos: Carbohydrates, Phytohormones and Proteomic Status
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SYSNO ASEP 0556744 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Desiccation as a Post-maturation Treatment Helps Complete Maturation of Norway Spruce Somatic Embryos: Carbohydrates, Phytohormones and Proteomic Status Author(s) Eliášová, Kateřina (UEB-Q) RID, ORCID
Konrádová, H. (CZ)
Dobrev, Petre (UEB-Q) RID, ORCID
Motyka, Václav (UEB-Q) RID, ORCID
Lomenech, A. M. (FR)
Fischerová, Lucie (UEB-Q) RID, ORCID
Lelu-Walter, M.A. (FR)
Vondráková, Zuzana (UEB-Q) RID, ORCID
Teyssier, C. (FR)Number of authors 9 Article number 823617 Source Title Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 13, FEB 14 (2022)Number of pages 25 s. Language eng - English Country CH - Switzerland Keywords desiccation tolerance ; phytohormones ; Picea abies (L.) Karst ; proteomics ; raffinose family oligosaccharides ; somatic embryogenesis OECD category Plant sciences, botany R&D Projects 7AMB12FR017 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA19-12262S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000761702900001 EID SCOPUS 85125344019 DOI 10.3389/fpls.2022.823617 Annotation Exposure of Norway spruce (Picea abies) somatic embryos and those of many other conifers to post-maturation desiccation treatment significantly improves their germination. An integration analysis was conducted to understand the underlying processes induced during the desiccation phase at the molecular level. Carbohydrate, protein and phytohormone assays associated with histological and proteomic studies were performed for the evaluation of markers and actors in this phase. Multivariate comparison of mature somatic embryos with mature desiccated somatic embryos and/or zygotic embryos provided new insights into the processes involved during the desiccation step of somatic embryogenesis. Desiccated embryos were characterized by reduced levels of starch and soluble carbohydrates but elevated levels of raffinose family oligosaccharides. Desiccation treatment decreased the content of abscisic acid and its derivatives but increased total auxins and cytokinins. The content of phytohormones in dry zygotic embryos was lower than in somatic embryos, but their profile was mostly analogous, apart from differences in cytokinin profiles. The biological processes “Acquisition of desiccation tolerance”, “Response to stimulus”, “Response to stress” and “Stored energy” were activated in both the desiccated somatic embryos and zygotic embryos when compared to the proteome of mature somatic embryos before desiccation. Based on the specific biochemical changes of important constituents (abscisic acid, raffinose, stachyose, LEA proteins and cruciferins) induced by the desiccation treatment and observed similarities between somatic and zygotic P. abies embryos, we concluded that the somatic embryos approximated to a state of desiccation tolerance. This physiological change could be responsible for the reorientation of Norway spruce somatic embryos toward a stage suitable for germination. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2023 Electronic address http://doi.org/10.3389/fpls.2022.823617
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