A New Model Trypanosomatid, Novymonas esmeraldas: Genomic Perception of Its Candidatus Pandoraea novymonadis Endosymbiont

0555255 - BC 2022 RIV US eng J - Journal Article
Zakharova, A. - Saura, A. - Butenko, Anzhelika - Podešvová, L. - Warmusova, S. - Kostygov, A.Y. - Nenarokova, Anna - Lukeš, Julius - Opperdoes, F. R. - Yurchenko, V.
A New Model Trypanosomatid, Novymonas esmeraldas: Genomic Perception of Its Candidatus Pandoraea novymonadis Endosymbiont.
mBio. Roč. 12, č. 4 (2021), č. článku e01606-21. ISSN 2161-2129. E-ISSN 2150-7511
R&D Projects: GA MŠMT(CZ) EF16_019/0000759; GA ČR(CZ) GA20-07186S; GA ČR(CZ) GA21-09283S; GA MŠMT(CZ) LL1601
Institutional support: RVO:60077344
Keywords : insect trypanosomatids * symbiotic bacterium * strigomonas-culicis * crithidia-deanei * evolution * leishmania * metabolism * parasite * expression * alignment * Trypanosomatidae * Leishmaniinae * bacterial endosymbiont * genomics * metabolism
OECD category: Microbiology
Impact factor: 7.786, year: 2021
Method of publishing: Open access
https://journals.asm.org/doi/10.1128/mBio.01606-21

The closest relative of human pathogen Leishmania, the trypanosomatid Novymonas esmeraldas, harbors a bacterial endosymbiont Candidatus Pandoraea novymonadis. Based on genomic data, we performed a detailed characterization of the metabolic interactions of both partners. While in many respects the metabolism of N. esmeraldas resembles that of other Leishmaniinae, the endosymbiont provides the trypanosomatid with heme, essential amino acids, purines, some coenzymes, and vitamins. In return, N. esmeraldas shares with the bacterium several nonessential amino acids and phospholipids. Moreover, it complements its carbohydrate metabolism and urea cycle with enzymes missing from the Ca. Pandoraea novymonadis genome. The removal of the endosymbiont from N. esmeraldas results in a significant reduction of the overall translation rate, reduced expression of genes involved in lipid metabolism and mitochondrial respiratory activity, and downregulation of several aminoacyl-tRNA synthetases, enzymes involved in the synthesis of some amino acids, as well as proteins associated with autophagy. At the same time, the genes responsible for protection against reactive oxygen species and DNA repair become signifi- cantly upregulated in the aposymbiotic strain of this trypanosomatid. By knocking out a component of its flagellum, we turned N. esmeraldas into a new model trypanosomatid that is amenable to genetic manipulation using both conventional and CRISPR-Cas9-mediated approaches.
Permanent Link: http://hdl.handle.net/11104/0329779