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Study of microbiocenosis of canine dental biofilms
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SYSNO ASEP 0547342 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Study of microbiocenosis of canine dental biofilms Author(s) Kacirova, J. (SK)
Madari, A. (SK)
Mucha, R. (SK)
Kolesár Fecskeová, Lívia (MBU-M)
Mujakić, Izabela (MBU-M) ORCID
Koblížek, Michal (MBU-M) RID, ORCID
Nemcová, R. (SK)
Madar, M. (SK)Article number 19776 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 11, č. 1 (2021)Number of pages 9 s. Language eng - English Country DE - Germany Keywords bacterial-flora ; oral-health ; plaque ; nov. ; cats ; dogs Subject RIV EE - Microbiology, Virology OECD category Microbiology Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000704088100089 EID SCOPUS 85116362746 DOI 10.1038/s41598-021-99342-5 Annotation Dental biofilm is a complex microbial community influenced by many exogenous and endogenous factors. Despite long-term studies, its bacterial composition is still not clearly understood. While most of the research on dental biofilms was conducted in humans, much less information is available from companion animals. In this study, we analyzed the composition of canine dental biofilms using both standard cultivation on solid media and amplicon sequencing, and compared the two approaches. The 16S rRNA gene sequences were used to define the bacterial community of canine dental biofilm with both, culture-dependent and culture-independent methods. After DNA extraction from each sample, the V3-V4 region of the 16S rRNA gene was amplified and sequenced via Illumina MiSeq platform. Isolated bacteria were identified using universal primers and Sanger sequencing. Representatives of 18 bacterial genera belonging to 5 phyla were isolated from solid media. Amplicon sequencing largely expanded this information identifying in total 284 operational taxonomic units belonging to 10 bacterial phyla. Amplicon sequencing revealed much higher diversity of bacteria in the canine dental biofilms, when compared to standard cultivation approach. In contrast, cultured representatives of several bacterial families were not identified by amplicon sequencing. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.nature.com/articles/s41598-021-99342-5
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