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Time lapse synchrotron IR chemical imaging for observing the acclimation of a single algal cell to CO2 treatment
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SYSNO ASEP 0546810 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Time lapse synchrotron IR chemical imaging for observing the acclimation of a single algal cell to CO2 treatment Author(s) Azarfar, G. (US)
Aboualizadeh, E. (US)
Ratti, S. (IT)
Olivieri, C. (IT)
Norici, A. (IT)
Nasse, M. J. (DE)
Giordano, Mario (MBU-M) ORCID
Hirschmugl, C. J. (US)Article number 13246 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 11, č. 1 (2021)Number of pages 10 s. Language eng - English Country DE - Germany Keywords transform infrared-spectroscopy ; carbon allocation ; marine diatom ; fringes ; phytoplankton ; elimination ; microscopy ; spectra Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA17-18917S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000670729700023 EID SCOPUS 85108678383 DOI 10.1038/s41598-021-92657-3 Annotation Algae are the main primary producers in aquatic environments and therefore of fundamental importance for the global ecosystem. Mid-infrared (IR) microspectroscopy is a non-invasive tool that allows in principle studying chemical composition on a single-cell level. For a long time, however, mid-infrared (IR) imaging of living algal cells in an aqueous environment has been a challenge due to the strong IR absorption of water. In this study, we employed multi-beam synchrotron radiation to measure time-resolved IR hyperspectral images of individual Thalassiosira weissflogii cells in water in the course of acclimation to an abrupt change of CO2 availability (from 390 to 5000 ppm and vice versa) over 75 min. We used a previously developed algorithm to correct sinusoidal interference fringes from IR hyperspectral imaging data. After preprocessing and fringe correction of the hyperspectral data, principal component analysis (PCA) was performed to assess the spatial distribution of organic pools within the algal cells. Through the analysis of 200,000 spectra, we were able to identify compositional modifications associated with CO2 treatment. PCA revealed changes in the carbohydrate pool (1200-950 cm(-1)), lipids (1740, 2852, 2922 cm(-1)), and nucleic acid (1160 and 1201 cm(-1)) as the major response of exposure to elevated CO2 concentrations. Our results show a local metabolism response to this external perturbation. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.nature.com/articles/s41598-021-92657-3
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