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Diclofenac Alters the Cell Cycle Progression of the Green Alga Chlamydomonas reinhardtii
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SYSNO ASEP 0546629 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Diclofenac Alters the Cell Cycle Progression of the Green Alga Chlamydomonas reinhardtii Author(s) Harshkova, D. (PL)
Liakh, I. (PL)
Bialevich, Vitali (MBU-M) RID
Ondrejmišková, Kamila (MBU-M)
Aksmann, A. (PL)
Bišová, Kateřina (MBU-M) RIDArticle number 1936 Source Title Cells. - : MDPI
Roč. 10, č. 8 (2021)Number of pages 15 s. Language eng - English Country CH - Switzerland Keywords non-steroidal anti-inflammatory drug ; diclofenac ; cell cycle ; Chlamydomonas reinhardtii Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA19-12607S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000688980300001 EID SCOPUS 85115041946 DOI 10.3390/cells10081936 Annotation The aim of the study was to verify the hypothesis that a potential cause of the phytotoxicity of diclofenac (DCF, a non-steroidal anti-inflammatory drug) is an effect of cell cycle progression. This research was conducted using synchronous cultures of a model organism, green alga Chlamydomonas reinhardtii. The project examined DCF effects on selected parameters that characterize cell cycle progression, such as cell size, attainment of commitment points, DNA replication, number of nuclei formed during cells division and morphology of cells in consecutive stages of the cell cycle, together with the physiological and biochemical parameters of algae cells at different stages. We demonstrated that individual cell growth remained unaffected, whereas cell division was delayed in the DCF-treated groups grown in continuous light conditions, and the number of daughter cells from a single cell decreased. Thus, the cell cycle progression is a target affected by DCF, which has a similar anti-proliferative effect on mammalian cells. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.mdpi.com/2073-4409/10/8/1936
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