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Biological Applications of Short Wavelength Microscopy Based on Compact, Laser-Produced Gas-Puff Plasma Source
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SYSNO ASEP 0538338 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Biological Applications of Short Wavelength Microscopy Based on Compact, Laser-Produced Gas-Puff Plasma Source Author(s) Torrisi, Alfio (UJF-V) RID, ORCID
Wachulak, P. W. (PL)
Bartnik, A. (PL)
Wegrzynski, L. (PL)
Fok, T. (PL)
Fiedorowicz, H. (PL)Number of authors 6 Article number 8338 Source Title Applied Sciences-Basel. - : MDPI
Roč. 10, č. 23 (2020)Number of pages 32 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords extreme ultraviolet ; laser-matter interaction ; microscopy Subject RIV BG - Nuclear, Atomic and Molecular Physics, Colliders OECD category Optics (including laser optics and quantum optics) Method of publishing Open access Institutional support UJF-V - RVO:61389005 UT WOS 000597110900001 EID SCOPUS 85096575011 DOI 10.3390/app10238338 Annotation Short wavelength compact microscopy setups, operating in the biological-dedicated 'water window' spectral range, perform sample imaging with a nanometer spatial resolution, employing complementary tools to the existing imaging techniques.
Over the last decades, remarkable efforts have been made to improve the resolution in photon-based microscopes. The employment of compact sources based on table-top laser-produced soft X-ray (SXR) in the 'water window' spectral range (lambda = 2.3-4.4 nm) and extreme ultraviolet (EUV) plasma allowed to overcome the limitations imposed by large facilities, such as synchrotrons and X-ray free electron lasers (XFEL), because of their high complexity, costs, and limited user access. A laser-plasma double stream gas-puff target source represents a powerful tool for microscopy operating in transmission mode, significantly improving the spatial resolution into the nanometric scale, comparing to the traditional visible light (optical) microscopes. Such an approach allows generating the plasma efficiently, without debris, providing a high flux of EUV and SXR photons. In this review, we present the development and optimization of desktop imaging systems: a EUV and an SXR full field microscope, allowing to achieve a sub-50 nm spatial resolution with short exposure time and an SXR contact microscope, capable to resolve internal structures in a thin layer of sensitive photoresist. Details about the source, as well as imaging results for biological applications, will be presented and discussed.Workplace Nuclear Physics Institute Contact Markéta Sommerová, sommerova@ujf.cas.cz, Tel.: 266 173 228 Year of Publishing 2021 Electronic address https://doi.org/10.3390/app10238338
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