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Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacterium
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SYSNO ASEP 0537030 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-1. Isolation and Characterization of the Bacterium Author(s) Pernicová, I. (CZ)
Nováčková, I. (CZ)
Sedláček, P. (CZ)
Kouřilová, X. (CZ)
Kalina, M. (CZ)
Kovalčik, A. (CZ)
Koller, M. (AT)
Nebesářová, Jana (BC-A) RID, ORCID
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Hrubanová, Kamila (UPT-D) RID, SAI, ORCID
Másílko, J. (CZ)
Slaninova, E. (CZ)
Obruča, S. (CZ)Number of authors 13 Article number 1235 Source Title Polymers. - : MDPI
Roč. 12, č. 6 (2020)Number of pages 13 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords waste glycerol ; poly(3-hydroxybutyrate) ; bacillus ; biosynthesis ; poly(3-hydroxybutyrate-co-3-hydroxyvalerate) ; polyhydroxyalkanoates ; thermophiles ; Aneurinibacillussp ; P(3HB-co-4HB) ; P(3HB-co-3HV-co-4HB) Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) Subject RIV - cooperation Institute of Scientific Instruments - Electronics ; Optoelectronics, Electrical Engineering R&D Projects LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BC-A - RVO:60077344 ; UPT-D - RVO:68081731 UT WOS 000550802300001 EID SCOPUS 85087631293 DOI 10.3390/polym12061235 Annotation Extremophilic microorganisms are considered being very promising candidates for biotechnological production of various products including polyhydroxyalkanoates (PHA). The aim of this work was to evaluate the PHA production potential of a novel PHA-producing thermophilic Gram-positive isolateAneurinibacillussp. H1. This organism was capable of efficient conversion of glycerol into poly(3-hydroxybutyrate) (P3HB), the homopolyester of 3-hydroxybutyrate (3HB). In flasks experiment, under optimal cultivation temperature of 45 degrees C, the P3HB content in biomass and P3HB titers reached 55.31% of cell dry mass and 2.03 g/L, respectively. Further, the isolate was capable of biosynthesis of PHA copolymers and terpolymers containing high molar fractions of 3-hydroxyvalerate (3HV) and 4-hydroxybutyrate (4HB). Especially 4HB contents in PHA were very high (up to 91 mol %) when 1,4-butanediol was used as a substrate. Based on these results, it can be stated thatAneurinibacillussp. H1 is a very promising candidate for production of PHA with tailored material properties. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2021 Electronic address https://www.mdpi.com/2073-4360/12/6/1235
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