Preparation of nanoliposomes by microfluidic mixing in herring-bone channel and the role of membrane fluidity in liposomes formation

0524856 - FZÚ 2021 RIV GB eng J - Journal Article
Kotouček, J. - Hubatka, F. - Mašek, J. - Kulich, P. - Velínská, K. - Bezděková, J. - Fojtíková, M. - Bartheldyová, E. - Tomečková, A. - Stráská, J. - Hrebík, D. - Macaulay, S. - Kratochvílová, Irena - Raška, M. - Turánek, J.
Preparation of nanoliposomes by microfluidic mixing in herring-bone channel and the role of membrane fluidity in liposomes formation.
Scientific Reports. Roč. 10, č. 1 (2020), s. 1-11, č. článku 5595. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA MŠMT(CZ) EF16_019/0000760
Grant - others:OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760
Institutional support: RVO:68378271
Keywords : nanoliposomes * microfluidic mixing
OECD category: Biophysics
Impact factor: 4.380, year: 2020
Method of publishing: Open access

Introduction of microfluidic mixing technique opens a new door for preparation of the liposomes and lipid-based nanoparticles by on-chip technologies that are applicable in a laboratory and industrial scale. This study demonstrates the role of phospholipid bilayer fragment as the key intermediate in the mechanism of liposome formation by microfluidic mixing in the channel with “herring-bone” geometry used with the instrument NanoAssemblr. The fluidity of the lipid bilayer expressed as fluorescence anisotropy of the probe N,N,N-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl) was found to be the basic parameter affecting the final size of formed liposomes prepared by microfluidic mixing of an ethanol solution of lipids and water phase. Both saturated and unsaturated lipids together with various content of cholesterol were used for liposome preparation and it was demonstrated, that an increase in fluidity results in a decrease of liposome size as analyzed by DLS.

Permanent Link: http://hdl.handle.net/11104/0309103