Preparation and Standardisation of Smoke-Water for Seed Germination and Plant Growth Stimulation

0524406 - ÚEB 2021 RIV US eng J - Journal Article
Gupta, S. - Hrdlička, Jakub - Ngoroyemoto, N. - Nemahunguni, N. K. - Gucký, Tomáš - Novák, Ondřej - Kulkarni, M. G. - Doležal, Karel - Van Staden, J.
Preparation and Standardisation of Smoke-Water for Seed Germination and Plant Growth Stimulation.
Journal of Plant Growth Regulation. Roč. 39, č. 1 (2020), s. 338-345. ISSN 0721-7595. E-ISSN 1435-8107
R&D Projects: GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : Grand Rapids * Karrikinolide * Smoke apparatus * Smoke-water * uhplc-esi(+)-ms/ms
OECD category: Biochemistry and molecular biology
Impact factor: 4.169, year: 2020
Method of publishing: Open access
http://doi.org/10.1007/s00344-019-09985-y

Smoke-water (SW) has a positive effect on seed germination in many plant species and its application in various fields of plant science has become popular. The method of preparing SW is relatively easy and inexpensive. However, many researchers working in the field of smoke biology are still unfamiliar with preparing SW and testing it for bioactivity. Thus, the aim of this study was to develop a low-cost efficient apparatus to produce SW using grasses, its standardisation and quantification of major active biomolecules. The prepared crude SW was diluted with distilled water (SW:DW) to 75:25, 50:50 and 25:75 (v/v) and these sub-dilutions were further diluted to 1:500 - 1:1000 - 1:1500 - 1:2000 - 1:2500 - 1:3000 and 1:3500 (v/v). For the standardisation of SW, lettuce seeds (Lactuca sativa L. cv. Grand Rapids) were tested to determine the best working concentration. The ratio 1:2500 (v/v) from the sub-dilution 25:75 (v/v) of SW was the best for germination of lettuce seeds in the dark, achieving 91% germination against the water control that had only 7–10% germination. This is the first study which reports the levels of stimulatory (karrikinolide 1, KAR1 and karrikinolide 2, KAR2) and inhibitory (trimethylbutenolide, TMB) compounds present in SW using ultrahigh-performance liquid chromatography–electrospray positive ionisation tandem mass spectrometry (UHPLC-ESI(+)-MS/MS).
Permanent Link: http://hdl.handle.net/11104/0308770