Developmental mechanisms driving complex tooth shape in reptiles

Landová Šulcová, Marie; Zahradníček, Oldřich; Dumková, J.; Dosedělová, Hana; Křivánek, J.; Hampl, Marek; Kavková, M.; Zikmund, T.; Gregorovičová, Martina; Sedmera, David; Kaiser, J.; Tucker, A. S.; Buchtová, Marcela

doi:https://dx.doi.org/10.1002/dvdy.138

Number of the records: 1

Developmental mechanisms driving complex tooth shape in reptiles

1.

SYSNO ASEP	0523882
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Developmental mechanisms driving complex tooth shape in reptiles
Author(s)	Landová Šulcová, Marie (UZFG-Y)_ORCID Zahradníček, Oldřich (UEM-P)_RID Dumková, J. (CZ) Dosedělová, Hana (UZFG-Y) Křivánek, J. (CZ) Hampl, Marek (UZFG-Y)_ORCID Kavková, M. (CZ) Zikmund, T. (CZ) Gregorovičová, Martina (FGU-C)_{RID, ORCID, SAI} Sedmera, David (FGU-C)_{RID, ORCID, SAI} Kaiser, J. (CZ) Tucker, A. S. (GB) Buchtová, Marcela (UZFG-Y)_{RID, ORCID}
Source Title	Developmental Dynamics. - : Wiley - ISSN 1058-8388 Roč. 249, č. 4 (2020), s. 441-464
Number of pages	24 s.
Publication form	Print - P
Language	eng - English
Country	US - United States
Keywords	chameleon ; gecko ; crocodile ; tooth shape ; SHH
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Developmental biology
Subject RIV - cooperation	Institute of Experimental Medicine - Genetics ; Molecular Biology
R&D Projects	EF15_003/0000460 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA17-14886S GA ČR - Czech Science Foundation (CSF)
	LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Research Infrastructure	Czech-BioImaging - 90062 - Ústav molekulární genetiky AV ČR, v. v. i.
Method of publishing	Limited access
Institutional support	UZFG-Y - RVO:67985904 ; UEM-P - RVO:68378041 ; FGU-C - RVO:67985823
UT WOS	000502529500001
EID SCOPUS	85076723246
DOI	10.1002/dvdy.138
Annotation	Background In mammals, odontogenesis is regulated by transient signaling centers known as enamel knots (EKs), which drive the dental epithelium shaping. However, the developmental mechanisms contributing to formation of complex tooth shape in reptiles are not fully understood. Here, we aim to elucidate whether signaling organizers similar to EKs appear during reptilian odontogenesis and how enamel ridges are formed. Results Morphological structures resembling the mammalian EK were found during reptile odontogenesis. Similar to mammalian primary EKs, they exhibit the presence of apoptotic cells and no proliferating cells. Moreover, expression of mammalian EK-specific molecules (SHH, FGF4, and ST14) and GLI2-negative cells were found in reptilian EK-like areas. 3D analysis of the nucleus shape revealed distinct rearrangement of the cells associated with enamel groove formation. This process was associated with ultrastructural changes and lipid droplet accumulation in the cells directly above the forming ridge, accompanied by alteration of membranous molecule expression (Na/K-ATPase) and cytoskeletal rearrangement (F-actin). Conclusions The final complex shape of reptilian teeth is orchestrated by a combination of changes in cell signaling, cell shape, and cell rearrangement. All these factors contribute to asymmetry in the inner enamel epithelium development, enamel deposition, ultimately leading to the formation of characteristic enamel ridges.
Workplace	Institute of Animal Physiology and Genetics
Contact	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Year of Publishing	2021
Electronic address	https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=74950514357

Number of the records: 1