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Intrinsic Electrocatalysis of RNA as a Label-free and Reagent-less Tool for Detection of MicroRNAs
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SYSNO ASEP 0511690 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Intrinsic Electrocatalysis of RNA as a Label-free and Reagent-less Tool for Detection of MicroRNAs Author(s) Římánková, Ludmila (BFU-R)
Ostatná, Veronika (BFU-R) RID, ORCID
Bartošík, M. (CZ)Number of authors 3 Source Title Electroanalysis. - : Wiley - ISSN 1040-0397
Roč. 31, č. 10 (2019), s. 1895-1900Number of pages 6 s. Publication form Print - P Language eng - English Country DE - Germany Keywords signal amplification strategy ; electrochemical determination ; stripping voltammetry ; cyclic voltammetry ; polarography ; chitosan ; microRNA Subject RIV CG - Electrochemistry OECD category Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 000491476600008 DOI 10.1002/elan.201900241 Annotation We show for the first time that RNA catalyzes hydrogen evolution reaction at mercury-containing electrodes. We previously showed that DNA is electrocatalytically active, and so we compared heights and potentials of the RNA chronopotentimetric stripping (CPS) peaks with analogous signals of DNA of the same sequence and found out they were very similar. RNA peaks showed differences depending on the RNA base composition. Catalytic nature of CPS peak enabled detection of 25 pM microRNA in electrochemical cell, or 500 pM microRNA in a 5 mu L solution drop (corresponding to 2.5 fmole of microRNA). This finding opens the door for simple, label-free and reagent-less analysis of low concentrations of RNA molecules. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2020 Electronic address https://onlinelibrary.wiley.com/doi/full/10.1002/elan.201900241
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