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Label-free Detection of Single Nucleotide Polymorphism Using Selective Incorporation of Biotin Anchor and Magnetoseperation on Streptavidin Magnetic Beads
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SYSNO ASEP 0506687 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Label-free Detection of Single Nucleotide Polymorphism Using Selective Incorporation of Biotin Anchor and Magnetoseperation on Streptavidin Magnetic Beads Author(s) Plucnara, Medard (BFU-R) ORCID
Havran, Luděk (BFU-R) RID, ORCID
Ecsin, E. (TR)
Erdem, A. (TR)
Fojta, Miroslav (BFU-R) RID, ORCIDNumber of authors 5 Source Title XXXVI. Moderní elektrochemické metody. Sborník přednášek. - Ústí nad Labem : Best servis, 2016 / Navrátil T. ; Fojta M. ; Schwarzová K. - ISBN 978-80-905221-4-5 Pages č. 2016 (2016), s. 161-165 Number of pages 5 s. Publication form Print - P Action Moderní elektrochemické metody /36./ Event date 23.05.2016 - 27.05.2016 VEvent location Jetřichovice Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords surface-plasmon resonance ; copper ions ; Label-free detection Subject RIV CF - Physical ; Theoretical Chemistry OECD category Physical chemistry R&D Projects GBP206/12/G151 GA ČR - Czech Science Foundation (CSF) Institutional support BFU-R - RVO:68081707 UT WOS 000389898200038 Annotation Novel approach of SNP detection in model synthetic oligonucleotides is described.This assay requires only intrinsic electrochemical properties of DNA and except of incorporation of biotin affinity tag, no any labeling or any other modification step is needed. Very good resolution for different variants of polymorphism as well as sufficiently low detection limits were achieved.In combination with using pencil graphited electrode this assay could be very simple and cheap tool for SNP typing. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2020
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