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Epitachophoretic separation and concentration of large volume samples
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SYSNO ASEP 0499153 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Epitachophoretic separation and concentration of large volume samples Author(s) Voráčová, Ivona (UIACH-O) RID, ORCID
Foret, František (UIACH-O) RID, ORCID
Datinská, Vladimíra (UIACH-O)
Novotný, Jakub (UIACH-O) ORCID
Gheibi, P. (US)
Berka, J. (US)
Astier, Y. (US)Number of authors 7 Source Title CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis. - Brno : Ústav analytické chemie AV ČR, v. v. i., 2018 / Foret F. ; Křenková J. ; Drobníková I. ; Klepárník K. ; Přikryl J. - ISBN 978-80-904959-5-1 Pages s. 316-319 Number of pages 4 s. Publication form Online - E Action CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./ Event date 15.10.2018 - 17.10.2018 VEvent location Brno Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords epitachophoretic separation ; DNA ; large sample volumes Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GBP206/12/G014 GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 Annotation There has been a growing interest in developing isotachophoretic protocols for concentration and purification of DNA as an alternative to the solid phase extraction protocols of DNA preparation. Also concentration of DNA from large volume samples is required. We have developed a new device suitable for separation, concentration and collection of ions from several milliliter sample volumes into microliter fractions. We have used a discontinuous electrolyte system comprising of the leading and terminating electrolytes utilized for concentration of samples containing DNA fragments. The method is simple, fast with high concentration factor and without extensive surface interactions. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2019 Electronic address http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf
Number of the records: 1