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Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis
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SYSNO ASEP 0489967 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis Author(s) Partyka, Jan (UIACH-O)
Křenková, Jana (UIACH-O) RID, ORCID
Čmelík, Richard (UIACH-O) RID
Foret, František (UIACH-O) RID, ORCIDNumber of authors 4 Source Title Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1560, JUL (2018), s. 91-96Number of pages 6 s. Publication form Print - P Language eng - English Country NL - Netherlands Keywords capillary electrophoresis ; hexahistidine ; labeling Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GJ16-09283Y GA ČR - Czech Science Foundation (CSF) GBP206/12/G014 GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 UT WOS 000434902900012 EID SCOPUS 85046873722 DOI 10.1016/j.chroma.2018.05.030 Annotation The labeling by amino acids and peptides was investigated for sensitive and fast analyses of oligosaccharides and N-linked glycans by capillary electrophoresis-mass spectrometry (CE-MS). Peptide tags with a various number of histidine residues were tested for maltooligosaccharide labeling in order to investigatethe effect of the size of labels and a number of charges on CE-MS analysis. Nevertheless, the reductive amination labeling of N-linked glycans by a hexahistidine tag resulted in a multiple products formation,therefore a peptide tag was modified by hydrazine functionality in order to perform labeling by hydra-zone formation chemistry. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2019
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