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Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis
- 1.0489967 - ÚIACH 2019 RIV NL eng J - Journal Article
Partyka, Jan - Křenková, Jana - Čmelík, Richard - Foret, František
Multi-charged labeling of oligosaccharides and N-linked glycans by hexahistidine-based tags for capillary electrophoresis-mass spectrometry analysis.
Journal of Chromatography A. Roč. 1560, JUL (2018), s. 91-96. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA ČR(CZ) GJ16-09283Y; GA ČR(CZ) GBP206/12/G014
Institutional support: RVO:68081715
Keywords : capillary electrophoresis * hexahistidine * labeling
OECD category: Analytical chemistry
Impact factor: 3.858, year: 2018
The labeling by amino acids and peptides was investigated for sensitive and fast analyses of oligosaccharides and N-linked glycans by capillary electrophoresis-mass spectrometry (CE-MS). Peptide tags with a various number of histidine residues were tested for maltooligosaccharide labeling in order to investigatethe effect of the size of labels and a number of charges on CE-MS analysis. Nevertheless, the reductive amination labeling of N-linked glycans by a hexahistidine tag resulted in a multiple products formation,therefore a peptide tag was modified by hydrazine functionality in order to perform labeling by hydra-zone formation chemistry.
Permanent Link: http://hdl.handle.net/11104/0284259
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