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In vitro evaluation of the monoclonal antibody Cu-64-IgG M75 against human carbonic anhydrase IX and its in vivo imaging
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SYSNO ASEP 0488728 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title In vitro evaluation of the monoclonal antibody Cu-64-IgG M75 against human carbonic anhydrase IX and its in vivo imaging Author(s) Čepa, Adam (UJF-V)
Ráliš, Jan (UJF-V) SAI
Král, Vlastimil (UMG-J) RID
Paurová, M. (CZ)
Kučka, Jan (UMCH-V) RID, ORCID
Humajová, J. (CZ)
Lázníček, M. (CZ)
Lebeda, Ondřej (UJF-V) RID, ORCID, SAINumber of authors 8 Source Title Applied Radiation and Isotopes. - : Elsevier - ISSN 0969-8043
Roč. 133, č. March (2018), s. 9-13Number of pages 5 s. Publication form Print - P Language eng - English Country GB - United Kingdom Keywords IgG M75 ; immunoaffinity ; imaging ; monoclonal atibody ; Cu-64 Subject RIV FP - Other Medical Disciplines OECD category Radiology, nuclear medicine and medical imaging Subject RIV - cooperation Institute of Molecular Genetics - Genetics ; Molecular Biology
Institute of Macromolecular Chemistry - Macromolecular ChemistryInstitutional support UJF-V - RVO:61389005 ; UMG-J - RVO:68378050 ; UMCH-V - RVO:61389013 UT WOS 000425577300003 EID SCOPUS 85038854401 DOI 10.1016/j.apradiso.2017.12.013 Annotation Specific oncology diagnostics requires new types of the selective radiophannaceuticals, particularly those suitable for the molecular PET imaging. The aim of this work is to present a new, specific PET-immunodiagnostic radiopharmaceutical based on the monoclonal antibody IgG M75 targeting human carbonic anhydrase IX labelled with Cu-64 (T-1/2 = 12.70 h) and its in vitro and in vivo evaluation. The antibody IgG M75 was conjugated with a non-commercial copper-specific chelator 'phosphinate' and then labelled with the positron emitter 64Cu. Stability of the labelled conjugated was tested in human serum. The immunoreactivity of the labelled conjugate was evaluated in vitro on a suitable cell cultures of the colorectal carcinoma (HT-29) and its imaging properties were estimated in vivo on a mouse model with inoculated colorectal carcinoma HT-29 imaged on a mu PET/CT. The tested radioimmunoconjugate was obtained in a specific activity of 0.25-0.5 MBq/mu g. In vitro uptake experiments revealed specific binding to the HT-29 cells (45 +/- 2.8% of the total added activity) and the measured K-D value was found to be 9.2 nM. Imaging clearly demonstrated significant uptake of the labelled monoclonal antibody in the tumour at 18 h post administration. The radioimmunoconjugate Cu-64-PS-IgG M75 seems to be a suitable candidate for PET diagnostics of hypoxic tumours expressing human carbonic anhydrase IX. Workplace Nuclear Physics Institute Contact Markéta Sommerová, sommerova@ujf.cas.cz, Tel.: 266 173 228 Year of Publishing 2019
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