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Genomic Analysis of the Evolution of Phototrophy among Haloalkaliphilic Rhodobacterales
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SYSNO ASEP 0479403 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Genomic Analysis of the Evolution of Phototrophy among Haloalkaliphilic Rhodobacterales Author(s) Kopejtka, Karel (MBU-M) ORCID, RID
Tomasch, J. (DE)
Zeng, Y. (DK)
Tichý, Martin (MBU-M) RID
Sorokin, D. Y. (RU)
Koblížek, Michal (MBU-M) RID, ORCIDSource Title Genome Biology and Evolution. - : Oxford University Press - ISSN 1759-6653
Roč. 9, č. 7 (2017), s. 1950-1962Number of pages 13 s. Language eng - English Country GB - United Kingdom Keywords Anoxygenic photosynthesis ; bacteriochlorophyll ; horizontal gene transfer Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GBP501/12/G055 GA ČR - Czech Science Foundation (CSF) LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support MBU-M - RVO:61388971 UT WOS 000410676100010 EID SCOPUS 85028405079 DOI 10.1093/gbe/evx141 Annotation A characteristic feature of the order Rhodobacterales is the presence of a large number of photoautotrophic and photoheterotrophic species containing bacteriochlorophyll. Interestingly, these phototrophic species are phylogenetically mixed with chemotrophs. To better understand the origin of such variability, we sequenced the genomes of three closely related haloalkaliphilic species, differing in their phototrophic capacity and oxygen preference: the photoheterotrophic and facultatively anaerobic bacterium Rhodobaca barguzinensis, aerobic photoheterotroph Roseinatronobacter thiooxidans, and aerobic heterotrophic bacteriumNatronohydrobacter thiooxidans. These three haloalcaliphilic species are phylogenetically related and share many common characteristics with the Rhodobacter species, forming together the Rhodobacter-Rhodobaca (RR) group. A comparative genomic analysis showed close homology of photosynthetic proteins and similarity in photosynthesis gene organization among the investigated phototrophic RR species. On the other hand, Rhodobaca barguzinensis and Roseinatronobacter thiooxidans lack an inorganic carbon fixation pathway and outer light-harvesting genes. This documents the reduction of their photosynthetic machinery towards a mostly photoheterotrophic lifestyle. Moreover, both phototrophic species contain 5-aminolevulinate synthase (encoded by the hemAgene) incorporated into their photosynthesis gene clusters, which seems to be a commonfeature of all aerobic anoxygenic phototrophic Alphaproteobacteria. Interestingly, the chrR-rpoE (sigma24) operon, which is part of singlet oxygen defense in phototrophic species, was found in the heterotrophic strain Natronohydrobacter thiooxidans. This suggests that this organism evolved from a photoheterotrophic ancestor through the loss of its photosynthesis genes. The overall evolution of phototrophy among the haloalkaliphilic members of the RR group is discussed. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2018
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