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Transcriptomic response of Arabidopsis thaliana (L.) Heynh. roots to ibuprofen
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SYSNO ASEP 0476651 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Transcriptomic response of Arabidopsis thaliana (L.) Heynh. roots to ibuprofen Author(s) Landa, Přemysl (UEB-Q) RID, ORCID
Přerostová, Sylva (UEB-Q) RID, ORCID, SAI
Langhansová, Lenka (UEB-Q) RID, ORCID
Maršík, Petr (UEB-Q) RID, ORCID
Vaněk, Tomáš (UEB-Q) RID, ORCIDNumber of authors 5 Source Title International Journal of Phytoremediation. - : Taylor & Francis - ISSN 1522-6514
Roč. 19, č. 8 (2017), s. 695-700Number of pages 6 s. Language eng - English Country US - United States Keywords detoxification ; gene expression ; metabolism of xenobiotics ; microarrays ; phytoremediation Subject RIV CE - Biochemistry OECD category Plant sciences, botany R&D Projects GA14-22593S GA ČR - Czech Science Foundation (CSF) Institutional support UEB-Q - RVO:61389030 UT WOS 000402041800001 EID SCOPUS 85019615193 DOI 10.1080/15226514.2016.1267697 Annotation Surface waters in urban areas are contaminated by ibuprofen (IBP), a popular and extensively used anti-inflammatory drug. In this study, we investigated the transcriptomic response in Arabidopsis thaliana (L.) Heynh. roots with the aim of revealing genes that are potentially involved in IBP detoxification and elucidating the effect of IBP on plants. IBP upregulated 63 and downregulated 38 transcripts (p-value < 0.1, fold change ≥2) after 2-day exposure to a 5-µM (1.03 mg/L) concentration of IBP under hydroponic conditions. Although the IBP concentration used in the experiment was highly relative to the concentrations found in rivers and wastewater, the number of genes with transcriptional changes was relatively low. The upregulation of cytochrome P450s, glutathione S-transferases, and UDP-glycosyltransferases indicates the occurrence of IBP oxidation in the first phase, followed by conjugation with glutathione and sugar in the second detoxification phase. ABC transporters could be involved in the transport of IBP and its metabolites. The identification of genes potentially involved in IBP detoxification could be useful in an IBP phytoremediation approach. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2018
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