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Effect of crosslinking chemistry of albumin/heparin multilayers on FGF-2 adsorption and endothelial cell behavior
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SYSNO ASEP 0473967 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Effect of crosslinking chemistry of albumin/heparin multilayers on FGF-2 adsorption and endothelial cell behavior Author(s) Kumorek, Marta M. (UMCH-V) RID
Janoušková, Olga (UMCH-V) RID, SAI, ORCID
Höcherl, Anita (UMCH-V) RID
Houska, Milan (UMCH-V) RID
Mázl Chánová, Eliška (UMCH-V) RID
Kasoju, Naresh (UMCH-V) RID, ORCID
Cuchalová, Lucie (UMCH-V) RID
Matějka, R. (CZ)
Kubies, Dana (UMCH-V) RID, ORCIDSource Title Applied Surface Science. - : Elsevier - ISSN 0169-4332
Roč. 411, 31 July (2017), s. 240-250Number of pages 11 s. Language eng - English Country NL - Netherlands Keywords basic fibroblast growth factor ; heparin ; cross-linking Subject RIV CD - Macromolecular Chemistry OECD category Polymer science R&D Projects LQ1604 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UMCH-V - RVO:61389013 UT WOS 000401391900030 EID SCOPUS 85016429973 DOI 10.1016/j.apsusc.2017.03.193 Annotation The biomaterials that efficiently deliver growth factors represent an important challenge in cell-based tissue engineering. Layer-by-layer (LbL) thin films are attractive for incorporating controlled amounts of growth factors and releasing them over time. Herein, we investigated the effect of a method of cross-linking of albumin/heparin layer-by-layer (LbL) assembly ((Alb/Hep)3) on the loading and release of basic fibroblast growth factor (FGF-2), and subsequent proliferation of human endothelial cells (HUVECs). The (Alb/Hep)3 assemblies were cross-linked using glutaraldehyde, reductive amination or carbodiimide chemistries, and then biofunctionalized with FGF-2. The (Alb/Hep)3 assemblies were characterized by the infrared multi-internal reflection spectroscopy, atomic force microscopy, ellipsometry, and surface plasmon resonance (SPR). The FGF-2 loading was quantified by the SPR in situ analysis. Our results showed that the (Alb/Hep)3 cross-linking affected the amount of the bound heparin (from 150 to 315 ng/cm2), amount of FGF-2 loaded (from 75 to 125 ng/cm2), FGF-2 release (from 15 to 53% over 8 days), and consequently the HUVEC cell proliferation (from 50 to 80 × 103 cells/cm2 at day 5). All FGF-2 loaded assemblies stimulated the cell growth more than a soluble FGF-2 added into the cell media. In particular, the highest HUVECs proliferation was detected on the carbodiimide-cross-linked assembly. Overall, these biocompatible cross-linked assemblies can fine-tune the loading and release of growth factor providing a platform for cell-contacting applications. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2018
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