Recent progress in electrochemical sensors and assays for DNA damage and repair

0471986 - BFÚ 2017 RIV GB eng J - Journal Article
Fojta, Miroslav - Daňhel, Aleš - Havran, Luděk - Vyskočil, V.
Recent progress in electrochemical sensors and assays for DNA damage and repair.
TrAC-Trends in Analytical Chemistry. Roč. 79, MAY2016 (2016), s. 160-167. ISSN 0165-9936. E-ISSN 1879-3142
R&D Projects: GA ČR GBP206/12/G151
Institutional support: RVO:68081707
Keywords : in-situ evaluation * carbon-paste electrode * voltammetric detection
Subject RIV: BO - Biophysics
Impact factor: 8.442, year: 2016

DNA in cells is permanently exposed to endogenous or exogenous chemical or physical agents which may cause chemical changes in the DNA structure collectively called "DNA damage", which may have severe impacts on human health. Therefore, efficient techniques to detect DNA damage are sought, among which electroanalytical methods hold an important position. This review is focused on recent progress in the field of electrochemical sensors and assays for DNA damage, particularly on typical applications of advanced electrochemical techniques in detecting oxidative DNA damage, abasic lesions, base mismatches, non-covalent interactions with drugs and other substances. Special attention is paid to combinations of electrochemical detection with biochemical tools such as enzymatic systems mimicking metabolic activation of xenobiotics, or DNA repair enzymes converting specific nucleobase lesions to species offering more facile electrochemical detection. These applications create conditions for application of electroanalytical approaches in biological research, including studies of DNA repair processes. (C) 2015 Elsevier B.V. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0269345