Number of the records: 1  

CyanoP is Involved in the Early Steps of Photosystem II Assembly in the Cyanobacterium Synechocystis sp PCC 6803

  1. 1.
    SYSNO ASEP0470774
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleCyanoP is Involved in the Early Steps of Photosystem II Assembly in the Cyanobacterium Synechocystis sp PCC 6803
    Author(s) Knoppová, Jana (MBU-M) RID, ORCID
    Yu, J. (GB)
    Koník, P. (CZ)
    Nixon, P. (GB)
    Komenda, Josef (MBU-M) RID, ORCID
    Source TitlePlant and Cell Physiology. - : Oxford University Press - ISSN 0032-0781
    Roč. 57, č. 9 (2016), s. 1921-1931
    Number of pages11 s.
    Languageeng - English
    CountryJP - Japan
    KeywordsCyanobacteria ; CyanoP ; Photosynthesis
    Subject RIVEE - Microbiology, Virology
    R&D ProjectsGBP501/12/G055 GA ČR - Czech Science Foundation (CSF)
    LM2015055 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Institutional supportMBU-M - RVO:61388971
    UT WOS000384717400011
    EID SCOPUS84988384578
    DOI https://doi.org/10.1093/pcp/pcw115
    AnnotationAlthough the PSII complex is highly conserved in cyanobacteria and chloroplasts, the PsbU and PsbV subunits stabilizing the oxygen-evolving Mn4CaO5 cluster in cyanobacteria are absent in chloroplasts and have been replaced by the PsbP and PsbQ subunits. There is, however, a distant cyanobacterial homolog of PsbP, termed CyanoP, of unknown function. Here we show that CyanoP plays a role in the early stages of PSII biogenesis in Synechocystis sp. PCC 6803. CyanoP is present in the PSII reaction center assembly complex (RCII) lacking both the CP47 and CP43 modules and binds to the smaller D2 module. A small amount of larger PSII core complexes co-purifying with FLAG-tagged CyanoP indicates that CyanoP can accompany PSII on most of its assembly pathway. A role in biogenesis is supported by the accumulation of unassembled D1 precursor and impaired formation of RCII in a mutant lacking CyanoP. Interestingly, the pull-down preparations of CyanoP-FLAG from a strain lacking CP47 also contained PsbO, indicating engagement of this protein with PSII at a much earlier stage in assembly than previously assumed.
    WorkplaceInstitute of Microbiology
    ContactEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Year of Publishing2017
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.