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Deciphering the relationship among phosphate dynamics, electron-dense body and lipid accumulation in the green alga Parachlorella kessleri
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SYSNO ASEP 0469267 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Deciphering the relationship among phosphate dynamics, electron-dense body and lipid accumulation in the green alga Parachlorella kessleri Author(s) Ota, S. (JP)
Yoshihara, M. (JP)
Yamazaki, T. (JP)
Takeshita, T. (JP)
Hirata, A. (JP)
Konomi, M. (JP)
Oshima, K. (JP)
Hattori, M. (JP)
Bišová, Kateřina (MBU-M) RID
Zachleder, Vilém (MBU-M) ORCID
Kawano, S. (JP)Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 6, MAY 16 (2016), s. 25731Number of pages 11 s. Language eng - English Country GB - United Kingdom Keywords electron-dense body ; lipid accumulation ; Parachlorella kessleri Subject RIV EE - Microbiology, Virology Institutional support MBU-M - RVO:61388971 UT WOS 000375899200001 EID SCOPUS 84984895732 DOI 10.1038/srep25731 Annotation Phosphorus is an essential element for life on earth and is also important for modern agriculture, which is dependent on inorganic fertilizers from phosphate rock. Polyphosphate is a biological polymer of phosphate residues, which is accumulated in organisms during the biological wastewater treatment process to enhance biological phosphorus removal. Here, we investigated the relationship between polyphosphate accumulation and electron-dense bodies in the green alga Parachlorella kessleri. Under sulfur-depleted conditions, in which some symporter genes were upregulated, while others were downregulated, total phosphate accumulation increased in the early stage of culture compared to that under sulfur-replete conditions. The P signal was detected only in dense bodies by energy dispersive X-ray analysis. Transmission electron microscopy revealed marked ultrastructural variations in dense bodies with and without polyphosphate. Our findings suggest that the dense body is a site of polyphosphate accumulation, and P. kessleri has potential as a phosphate-accumulating organism. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2017
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