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The Assembly Factor SDHAF2 Is Dispensable for Flavination of the Catalytic Subunit of Mitochondrial Complex II in Breast Cancer Cells
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SYSNO ASEP 0468544 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Assembly Factor SDHAF2 Is Dispensable for Flavination of the Catalytic Subunit of Mitochondrial Complex II in Breast Cancer Cells Author(s) Bezawork-Geleta, A. (AU)
Dong, L. (AU)
Rohlena, Jakub (BTO-N) RID, ORCID
Neužil, Jiří (BTO-N) RIDNumber of authors 4 Source Title Journal of Biological Chemistry. - : Elsevier - ISSN 0021-9258
Roč. 291, č. 41 (2016), s. 21414-21420Number of pages 7 s. Language eng - English Country US - United States Keywords cancer biology ; mitochondrial respiratory chain complex ; SUCCINATE-UBIQUINONE OXIDOREDUCTASE Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA15-02203S GA ČR - Czech Science Foundation (CSF) GA16-22823S GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BTO-N - RVO:86652036 UT WOS 000385999200007 DOI 10.1074/jbc.C116.755017 Annotation Mitochondrial complex II or succinate dehydrogenase (SDH) is at the crossroads of oxidative phosphorylation and the tricarboxylic acid cycle. It has been shown that Sdh5 (SDHAF2/SDH5 in mammals) is required for flavination of the subunit Sdh1 (SDHA in human cells) in yeast. Here we demonstrate that in human breast cancer cells, SDHAF2/SDH5 is dispensable for SDHA flavination. In contrast to yeast, CRISPR-Cas9 nickase-mediated SDHAF2 KO breast cancer cells feature flavinated SDHA and retain fully assembled and functional complex II, as well as normal mitochondrial respiration. Our data show that SDHA flavination is independent of SDHAF2 in breast cancer cells, employing an alternative mechanism. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2017
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