Chromosome and Genomic Engineering in Plants

0464539 - ÚEB 2017 RIV US eng M - Monography Chapter
Cápal, Petr - Vrána, Jan - Kubaláková, Marie - Endo, T. R. - Doležel, Jaroslav
Chromosomal Allocation of DNA Sequences in Wheat Using Flow-Sorted Chromosomes.
Chromosome and Genomic Engineering in Plants. Vol. 1469. New York: Humana Press, 2016 - (Murata, M.), s. 157-170. Methods and Protocols, Methods in Molecular Biology. ISBN 978-1-4939-4929-8
R&D Projects: GA MŠMT(CZ) LO1204; GA ČR GBP501/12/G090
Institutional support: RVO:61389030
Keywords : Chromosomal allocation * Chromosome genomics * Chromosome sorting
Subject RIV: EB - Genetics ; Molecular Biology

Flow cytometry enables chromosomes to be sorted into different groups based on their characteristics, such as relative DNA content and the presence of repetitive DNA sequences. Despite the recent progress in the analysis of plant genome organization and chromosome structure, there is a need for easy methods to assign DNA sequences to individual chromosomes. Here, we describe an easy way to allocate genes or DNA sequences to chromosomes in wheat using flow-sorted chromosomes combined with fluorescence in situ hybridization and PCR analyses.
Permanent Link: http://hdl.handle.net/11104/0263408