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Raman and fluorescence microscopy sensing energy-transducing and energy-storing structures in microalgae
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SYSNO ASEP 0463694 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Raman and fluorescence microscopy sensing energy-transducing and energy-storing structures in microalgae Author(s) Moudříková, Š. (CZ)
Mojzeš, P. (CZ)
Zachleder, Vilém (MBU-M) ORCID
Pfaff, Ch. (DE)
Behrendt, D. (DE)
Nedbal, L. (CZ)Source Title Algal Research-Biomass Biofuels and Bioproducts. - : Elsevier - ISSN 2211-9264
Roč. 16, June (2016), s. 224-232Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords Biofuels ; Lipids ; Photosynthesis Subject RIV EE - Microbiology, Virology Institutional support MBU-M - RVO:61388971 UT WOS 000375610000026 EID SCOPUS 84961129437 DOI 10.1016/j.algal.2016.03.016 Annotation Chlorophylls and carotenoids are core components of photosynthetic energy transduction in algal cells, whereas starch, lipids, and polyphosphates represent energy reserves. All these biomolecules exhibit characteristic molecular vibrations that were sensed and localized in individual cells of Desmodesmus quadricauda and Chlorella vulgaris by confocal Raman microscopy. In the same cells, fluorescence of chlorophylls and of lipid bodies stained with Nile Red was mapped using the same instrument. In the first of the three consecutive scans, a low-power and short-exposure excitation by a green 532 nm laser was used to map chlorophyll fluorescence. In the second scan, the full power of a 785 nm laser was used for Raman mapping of carotenoids. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2017
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