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Ready at the right time - challenges and milestones of reproduction
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SYSNO ASEP 0463150 Document Type C - Proceedings Paper (int. conf.) R&D Document Type The record was not marked in the RIV Title Ready at the right time - challenges and milestones of reproduction Author(s) Dvořáková-Hortová, Kateřina (BTO-N)
Frolíková, Michaela (BTO-N) ORCID
Děd, Lukáš (BTO-N) RID
Šebková, Nataša (BTO-N)Number of authors 4 Source Title Hauptprogramm 28. Jahrestagung der Deutschen Gesellschaft für Andrologie. - Saarbrücken : Deutsche Gesellschaft für Andrologie e.V, 2016 / Derouet Harry ; Behre Hermann M. Action 28. Jahrestagung der Deutschen Gesellschaft für Andrologie Event date 08.09.2016-10.09.2016 VEvent location Saarbrücken Country DE - Germany Event type WRD Language eng - English Country DE - Germany Keywords sperm-egg interaction ; IZUMO 1 ; STED ; CD46 ; sperm capacitation Subject RIV EC - Immunology R&D Projects GA14-05547S GA ČR - Czech Science Foundation (CSF) ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support BTO-N - RVO:86652036 Annotation Not every sperm can fertilize the egg, and the competition among male gametes is fierce. In order to become the chosen one, sperm must fulfil hundreds of tasks without making a single mistake. One of their crucial goals is to relocalize several proteins, which are necessary for sperm-egg interaction, binding and fusion. The dynamic protein network rearrangement already starts during the sperm capacitation and it is completed through the acrosome reaction. Our aim was to investigate the relationship between the primary fusion protein IZUMO1 and the tetraspanin proteins CD46 and β1-integrin. The molecular interaction of CD46 and β1-integrin was proven by using the Proximity Ligation Assay and Super resolution microscopy STED. Sperm that successfully accomplish the repositioning of the crucial proteins in a given time frame certainly gain a selective advantage over the rest of the sperm population. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2017
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