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Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21 (DE3) carrying a synthetic metabolic pathway
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SYSNO ASEP 0456331 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21 (DE3) carrying a synthetic metabolic pathway Author(s) Dvořák, P. (CZ)
Chrást, L. (CZ)
Nikel, P. (ES)
Fedr, Radek (BFU-R) ORCID
Souček, Karel (BFU-R) RID, ORCID
Sedláčková, M. (CZ)
Chaloupková, R. (CZ)
Lorenzo, V. (ES)
Prokop, Z. (CZ)
Damborský, J. (CZ)Number of authors 10 Source Title Microbial Cell Factories. - : BioMed Central
Roč. 14, č. 201 (2015)Number of pages 15 s. Publication form Online - E Language eng - English Country GB - United Kingdom Keywords Metabolic burden ; Substrate toxicity ; Escherichia coli Subject RIV BO - Biophysics Institutional support BFU-R - RVO:68081707 UT WOS 000367047300002 DOI 10.1186/s12934-015-0393-3 Annotation Background: Heterologous expression systems based on promoters inducible with isopropyl-beta-D-1-thiogalactopyranoside (IPTG), e.g., Escherichia coli BL21 (DE3) and cognate LacI(Q)/P-lacUV5-T7 vectors, are commonly used for production of recombinant proteins and metabolic pathways. The applicability of such cell factories is limited by the complex physiological burden imposed by overexpression of the exogenous genes during a bioprocess. This burden originates from a combination of stresses that may include competition for the expression machinery, side-reactions due to the activity of the recombinant proteins, or the toxicity of their substrates, products and intermediates. However, the physiological impact of IPTG-induced conditional expression on the recombinant host under such harsh conditions is often overlooked. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2016
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