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Interactions of fluorescent dye SYBR Green I with natural and 7-deazaguanine-modified DNA studied by fluorescence and electrochemical methods
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SYSNO ASEP 0456297 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Interactions of fluorescent dye SYBR Green I with natural and 7-deazaguanine-modified DNA studied by fluorescence and electrochemical methods Author(s) Dudová, Zdenka (BFU-R)
Špaček, Jan (BFU-R) ORCID
Havran, Luděk (BFU-R) RID, ORCID
Pivoňková, Hana (BFU-R) ORCID
Fojta, Miroslav (BFU-R) RID, ORCIDNumber of authors 5 Source Title Monatshefte fur Chemie. - : Springer - ISSN 0026-9247
Roč. 147, č. 1 (2016), s. 13-20Number of pages 8 s. Publication form Print - P Language eng - English Country AT - Austria Keywords 7-Deazaguanine ; PCR ; Fluorescence Subject RIV BO - Biophysics Institutional support BFU-R - RVO:68081707 UT WOS 000367521400003 DOI 10.1007/s00706-015-1578-5 Annotation YBR Green I (SG) is a fluorescent dye applied in various techniques of DNA analysis, including fluorescent staining of electrophoretic gels, quantitative polymerase chain reaction, etc. SG binds selectively to double-stranded DNA via intercalation and minor groove interactions, resulting in a considerable enhancement of fluorescence of the dye. Modification of DNA by partial or full replacement of natural purine nucleobase guanine (G) with its synthetic analog 7-deazaguanine (G*) or its derivatives was shown to cause the SG fluorescence quenching. In this paper, we present a comparative study of interactions of SG with natural DNA fragments and with DNA fragments modified with G* by means of fluorescence and electrochemical methods. Competition between unmodified (forming strongly fluorescent complex with SG) and fully G*-modified (not contributing significantly to overall fluorescence signal) DNA fragments for the dye was studied via changes in the fluorescence intensity. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2016
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