Asymmetric dimethyl arginine induces pulmonary vascular dysfunction via activation of signal transducer and activator of transcription 3 and stabilization of hypoxia-inducible factor 1-alpha

Pekarová, Michaela; Koudelka, Adolf; Kolářová, Hana; Ambrožová, Gabriela; Klinke, A.; Černá, A.; Kadlec, J.; Trundová, Mária; Šindlerová, Lenka; Kuchta, R.; Kuchtová, Z.; Lojek, Antonín; Kubala, Lukáš

doi:https://dx.doi.org/10.1016/j.vph.2015.06.005

Number of the records: 1

Asymmetric dimethyl arginine induces pulmonary vascular dysfunction via activation of signal transducer and activator of transcription 3 and stabilization of hypoxia-inducible factor 1-alpha

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SYSNO ASEP	0455893
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Asymmetric dimethyl arginine induces pulmonary vascular dysfunction via activation of signal transducer and activator of transcription 3 and stabilization of hypoxia-inducible factor 1-alpha
Author(s)	Pekarová, Michaela (BFU-R)_RID Koudelka, Adolf (BFU-R)_ORCID Kolářová, Hana (BFU-R)_RID Ambrožová, Gabriela (BFU-R)_RID Klinke, A. (DE) Černá, A. (CZ) Kadlec, J. (CZ) Trundová, Mária (BTO-N) Šindlerová, Lenka (BFU-R)_{RID, ORCID} Kuchta, R. (CZ) Kuchtová, Z. (CZ) Lojek, Antonín (BFU-R)_RID Kubala, Lukáš (BFU-R)_{RID, ORCID}
Number of authors	13
Source Title	Vascular Pharmacology. - : Elsevier - ISSN 1537-1891 Roč. 73, OCT 2015 (2015), s. 138-148
Number of pages	11 s.
Publication form	Print - P
Language	eng - English
Country	CZ - Czech Republic
Keywords	NITRIC-OXIDE PRODUCTION ; SMOOTH-MUSCLE-CELLS ; ARTERIAL-HYPERTENSION
Subject RIV	BO - Biophysics
Subject RIV - cooperation	Institute of Biotechnology - Biotechnology ; Bionics
R&D Projects	GP13-40882P GA ČR - Czech Science Foundation (CSF)
	EE2.3.30.0030 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	BFU-R - RVO:68081707
UT WOS	000362056600018
DOI	10.1016/j.vph.2015.06.005
Annotation	Pulmonary hypertension (PH), associated with imbalance in vasoactive mediators and massive remodeling of pulmonary vasculature, represents a serious health complication. Despite the progress in treatment, PH patients typically have poor prognoses with severely affected quality of life. Asymmetric dimethyl arginine (ADMA), endogenous inhibitor of endothelial nitric oxide synthase (eNOS), also represents one of the critical regulators of pulmonary vascular functions. The present study describes a novel mechanism of ADMA-induced dysfunction in human pulmonary endothelial and smooth muscle cells. The effect of ADMA was compared with well-established model of hypoxia-induced pulmonary vascular dysfunction. It was discovered for the first time that ADMA induced the activation of signal transducer and activator of transcription 3 (STAT3) and stabilization of hypoxia inducible factor la (HIF-1 alpha) in both types of cells, associated with drastic alternations in normal cellular functions (e.g., nitric oxide production, cell proliferation/Ca2+ concentration, production of pro-inflammatory mediators, and expression of eNOS, DDAH1, and ICAM-1).
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2016

Number of the records: 1