Characterization of cry1Cb3 and cry1Fb7 from Bacillus thuringiensis subsp. galleriae

0451085 - BC 2016 RIV PL eng J - Journal Article
Huang, T. - Xiao, Y. - Pan, J. - Zhang, L. - Gelbič, Ivan - Guan, X.
Characterization of cry1Cb3 and cry1Fb7 from Bacillus thuringiensis subsp. galleriae.
Open Life Sciences. Roč. 10, č. 1 (2015), s. 521-528. ISSN 2391-5412
Institutional support: RVO:60077344
Keywords : Bacillus thuringiensis subsp. galleriae * PCR-RFLP * cloning
Subject RIV: EB - Genetics ; Molecular Biology
http://www.degruyter.com/view/j/biol.2015.10.issue-1/biol-2015-0054/biol-2015-0054.xml

Two cry1-type genes encoding insecticidal crystal proteins (ICPs) were detected by PCR-RFLP and cloned from Bacillus thuringiensis subsp. galleriae 87. The nukleotide sequences were deposited in GenBank with accession numbers EU679501 and EU679502, and designated as cry1Fb7 and cry1Cb3 respectively by B. thuringiensis Delta-Endotoxin Nomenclature Committee. cry1Cb3 shared 99% homology with other cry1Cb genes. The existence of two additional stop codons indicated cry1Cb3 was a silent gene. The cry1Cb3 was 3531 bp with 38.98% G+C content and its first open reading frame (ORF) encoded a protein of 213 amino acid residues with a calculated molecular weight of 23.8 kDa and a predicted pI value of 4.63. Five amino acid sequence blocks (block 1, block 2, block 3, block 4 and block 5) were found in Cry1Cb3.
Permanent Link: http://hdl.handle.net/11104/0257736