The Extreme Anterior Domain Is an Essential Craniofacial Organizer Acting through Kinin-Kallikrein Signaling

0441368 - BTÚ 2015 RIV US eng J - Journal Article
Jacox, L. - Šindelka, Radek - Chen, J. - Rothman, A. - Dickinson, A. - Sive, H.
The Extreme Anterior Domain Is an Essential Craniofacial Organizer Acting through Kinin-Kallikrein Signaling.
Cell Reports. Roč. 8, č. 2 (2014), s. 595-608. ISSN 2211-1247. E-ISSN 2211-1247
Institutional support: RVO:86652036
Keywords : CRANIAL NEURAL CREST * NITRIC-OXIDE SYNTHASE * XENOPUS-LAEVIS
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 8.358, year: 2014

The extreme anterior domain (EAD) is a conserved embryonic region that includes the presumptive mouth. We show that the Kinin-Kallikrein pathway is active in the EAD and necessary for craniofacial development in Xenopus and zebrafish. The mouth failed to form and neural crest (NC) development and migration was abnormal after loss of function (LOF) in the pathway genes kng, encoding Bradykinin (xBdk), carboxypeptidase-N (cpn), which cleaves Bradykinin, and neuronal nitric oxide synthase (nNOS). Consistent with a role for nitric oxide (NO) in face formation, endogenous NO levels declined after LOF in pathway genes, but these were restored and a normal face formed after medial implantation of xBdk-beads into LOF embryos. Facial transplants demonstrated that Cpn function from within the EAD is necessary for the migration of first arch cranial NC into the face and for promoting mouth opening. The study identifies the EAD as an essential craniofacial organizer acting through Kinin-Kallikrein signaling.
Permanent Link: http://hdl.handle.net/11104/0244413