Effect of His(6)-tagging of anterior gradient 2 protein on its electro-oxidation

Ostatná, Veronika; Vargová, Veronika; Hrstka, R.; Durech, M.; Vojtešek, B.; Paleček, Emil

doi:https://dx.doi.org/10.1016/j.electacta.2014.10.125

Number of the records: 1

Effect of His(6)-tagging of anterior gradient 2 protein on its electro-oxidation

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SYSNO ASEP	0440148
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Effect of His(6)-tagging of anterior gradient 2 protein on its electro-oxidation
Author(s)	Ostatná, Veronika (BFU-R)_{RID, ORCID} Vargová, Veronika (BFU-R) Hrstka, R. (CZ) Durech, M. (CZ) Vojtešek, B. (CZ) Paleček, Emil (BFU-R)_{RID, ORCID}
Number of authors	6
Source Title	Electrochimica acta. - : Elsevier - ISSN 0013-4686 Roč. 150, DEC2014 (2014), s. 218-222
Number of pages	5 s.
Publication form	Print - P
Language	eng - English
Country	GB - United Kingdom
Keywords	Anterior Gradient 2?oncoprotein ; His-tagged proteins ; carbon electrodes
Subject RIV	BO - Biophysics
R&D Projects	GA13-00956S GA ČR - Czech Science Foundation (CSF)
Institutional support	BFU-R - RVO:68081707
UT WOS	000346185000028
DOI	10.1016/j.electacta.2014.10.125
Annotation	His-tagged and non-tagged forms of Anterior Gradient 2 (AGR2), oncoprotein and potential cancer biomarker, were studied for the first time using voltammetry at carbon electrodes. In addition to oxidation peak of Tyr and Trp, N-terminal His-tagged AGR2 form yielded characteristic electro-oxidation peak of histidine. Qualitatively similar results were obtained with other N-terminal His-tagged proteins, such as, glutathione-S-transferase, alpha-synuclein and cytochrome b5. Our results suggested that His-tag modification of proteins (commonly used in recombinant protein preparation) may change adsorption and orientation of the proteins at electrode surfaces. In absence of the His-tag in His-containing proteins, appearance of His peak was influenced by accessibility of His residues, and depended on the carbon electrode type. Oxidation His peak, in combination with Tyr and Trp oxidation responses may find use in label-free analysis of numerous proteins, including those important in biomedicine. (C) 2014 Elsevier Ltd. All rights reserved.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2015

Number of the records: 1