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Reproducible and time-course study of yeast biofilm by Raman spectroscopy
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SYSNO ASEP 0437746 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Reproducible and time-course study of yeast biofilm by Raman spectroscopy Author(s) Samek, Ota (UPT-D) RID, ORCID, SAI
Mlynariková, K. (CZ)
Bernatová, Silvie (UPT-D) RID, SAI
Ježek, Jan (UPT-D) RID, ORCID, SAI
Krzyžánek, Vladislav (UPT-D) RID, ORCID, SAI
Šiler, Martin (UPT-D) RID, ORCID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Růžička, F. (CZ)Number of authors 8 Source Title 19th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics (Proceedings of SPIE 9441). - Bellingham : SPIE, 2014 - ISSN 0277-786X - ISBN 9781628415568 Pages 94411m:1-9 Number of pages 9 s. Publication form Print - P Action Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics /19./ Event date 08.09.2014-12.09.2014 VEvent location Jelenia Góra Country PL - Poland Event type EUR Language eng - English Country US - United States Keywords Raman spectroscopy ; yeast ; chemometrics ; diagnostics ; principal component analysis Subject RIV BH - Optics, Masers, Lasers R&D Projects GAP205/11/1687 GA ČR - Czech Science Foundation (CSF) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 UT WOS 000349332600057 DOI 10.1117/12.2176031 Annotation We report on Raman spectroscopy measurements - separated by a given time intervals - for the selected yeast strains (biofilm positive and biofilm negative) on colonies grown directly on the Petri dishes or on the well-plate. Chemometric principal component analysis of these spectra sets generated clusters of data points, from which the reproducibility of the measurement could be analysed. Consequently, these resulted in clusters coinciding well with the biofilm positive and biofilm negative strains measurement of a particular sample dish, suggesting good reproducibility of our measurement procedure, even when the samples were prepared and measured days up to months apart. This suggests the potential of Raman spectroscopy in routine clinical diagnostic. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2015
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