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Raman tweezers in microfluidic systems for analysis and sorting of living cells
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SYSNO ASEP 0434961 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Raman tweezers in microfluidic systems for analysis and sorting of living cells Author(s) Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Ježek, Jan (UPT-D) RID, ORCID, SAI
Kaňka, Jan (UPT-D) RID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCIDNumber of authors 4 Source Title Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII (Proceedings of SPIE 8947). - Bellingham : SPIE, 2014 - ISSN 1605-7422 - ISBN 9780819498601 Pages 89471m:1-9 Number of pages 9 s. Publication form Print - P Action Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues /12./ Event date 03.02.2014-06.02.2014 VEvent location San Francisco Country US - United States Event type WRD Language eng - English Country US - United States Keywords Chemicals ; Fluorescent markers ; Optical analysis ; Optical trapping ; Organisms ; Raman spectroscopy Subject RIV BH - Optics, Masers, Lasers R&D Projects FR-TI1/433 GA MPO - Ministry of Industry and Trade (MPO) TA03010642 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) LO1212 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 UT WOS 000336037200037 EID SCOPUS 84901769335 DOI 10.1117/12.2040631 Annotation We have devised an analytical and sorting system combining optical trapping with Raman spectroscopy in microfluidic environment in order to identify and sort biological objects, such as living cells of various prokaryotic and eukaryotic organisms. Our main objective was to create a robust and universal platform for non-contact sorting of microobjects based on their Raman spectral properties. This approach allowed us to collect information about the chemical composition of the objects, such as the presence and composition of lipids, proteins, or nucleic acids without using artificial chemical probes such as fluorescent markers. The non-destructive and non-contact nature of this optical analysis and manipulation allowed us to separate individual living cells of our interest in a sterile environment and provided the possibility to cultivate the selected cells for further experiments. We used differently treated cells of algae to test and demonstrate the function of our analytical and sorting system. The devised system could find its use in many medical, biotechnological, and biological applications. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2015
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