0433022 - MBÚ 2015 RIV US eng J - Journal Article
Šírová, Milada - van Vlierberghe, S. - Matyášová, V. - Rossmann, Pavel - Schacht, E. - Dubruel, P. - Říhová, Blanka
Immunocompatibility evaluation of hydrogel-coated polyimide implants for applications in regenerative medicine.
Journal of Biomedical Materials Research. Part A. Roč. 102, č. 6 (2014), s. 1982-1990. ISSN 1549-3296. E-ISSN 1552-4965
R&D Projects: GA ČR GAP301/12/1254
Institutional support: RVO:61388971
Keywords : methacrylamide-modified gelatin * hydrogel * polyimide implant
Subject RIV: EC - Immunology
Impact factor: 3.369, year: 2014

Immunocompatibility of gelatin-based hydrogels to be applied as implant coatings for local regenerative treatment has been studied. First, the bio- and immuno-acceptability of the methacrylamide-modified gelatin hydrogels per se was screened. The results indicated that the hydrogels support cell growth. Metabolic activity of normal cells and permanent cell lines representing various cell types (endothelial, epithelial, fibroblast, and monocyte/macrophage) cultivated on the gelatin hydrogels was moderately lower compared to cells cultivated on tissue culture plastic. The cells cultivated on the hydrogels produced identical cytokines as the control cells although at lower levels. Importantly, no inflammatory activity, measured by nitric oxide and pro-inflammatory cytokine (IL-1, IL-6, and TNF) production, was observed in peritoneal cells and monocyte/macrophage RAW 264.7 cell line cultivated on the hydrogels
Permanent Link: http://hdl.handle.net/11104/0237346